| Project/Area Number |
26670004
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
Sasaki Shigeki 九州大学, 薬学研究科(研究院), 教授 (10170672)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ８－オキソグアノシン / ８－オキソグアノシントリリン酸 / ８－ニトログアノシン / ８－チオグアノシン / 共有結合的捕捉 / 認識分子 / 蛍光検出 / 酸化損傷塩基 / 活性酸素 / 活性窒素 / ８－オキソグアニン / ８－ニトログアニン / ８－チオグアニン / 選択的捕捉分子 |
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| Outline of Final Research Achievements |
8-Oxidized guanosine derivatives such as 8-oxo-, 8-nitro-, 8-thio- or 8-halo-2’-deoxyguanosine are markers of redox stage of cells. In this study, we have attempted to develop new recognition molecules for these 8-oxidized guanosine derivatives. In an approach, silica gels were chemically modified with 8-oxo-G clamp and were shown to selectively detect 8-oxo-dG in water.Artificial receptor molecules for 8-oxo-dG triphosphate have been successfully developed by connecting the cyclen-zinc complex with the phenoxazine skeleton.In particular, the linker structure between the cyclen-zinc complex and the phenoxazine skeleton play an important role for selectivity for 8-oxo-dG triphosphate.Development of selective molecules for covalent capture of 8-nitro-2’-deoxyguanoine was also successful using the thiol-containing molecules named 8-nitroG grasp. The chloride leaving group was introduced to the same skeleton instead of the thiol group, and shown to covalently capture 8-thio-dG.
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