Intracellular roles of 2-acyl-lysophospholipids

• Project/Area Number: 26670021
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Aoki Junken 東北大学, 薬学研究科(研究院), 教授 (20250219)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: リゾリン脂質 / 脂肪酸 / ２－アシルリゾリン脂質 / LC-MS/MS / ホスホリパーゼA1 / 2-アシル型リゾリン脂質 / 質量分析計

Outline of Final Research Achievements

Lysophospholipid is a kind of phospholipids with only one fatty acid either at the sn-1 or sn-2 position of glycerol. In contrast to the extracellular lysophospholipids, roles of intracellular lysophospholipids remain to be determined. In this study we tried to elucidate the role of intracellular lysophospholipids as well as its producing enzymes, intracellular phospholipase A1 (iPLA1). We found that, by inhibiting lysophospholipid acylation reaction, lysophospholipid level increased in cells. In addition, by over expressing iPLA1a, we increased the lysophospholipid level dramatically in the cells. Interestingly, we observed abnormal mitochondria and nuclear envelope in such cells, indicating possible role of intracellular lysophospholipids in regulating maintenance of these organelles.

Research Products

• [Journal Article] Possible involvement of minor lysophospholipids in the increase in plasma lysophosphatidic acid in acute coronary syndrome. (2015)
  • Author(s): Kurano M, Suzuki A, Inoue A, Tokuhara Y, Kano K, Matsumoto H, Igarashi K, Ohkawa R, Nakamura K, Dohi T, Miyauchi K, Daida H, Tsukamoto K, Ikeda H, Aoki J, Yatomi Y
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 35 Issue: 2 Pages: 463-470
  • DOI: 10.1161/atvbaha.114.304748
  • Peer Reviewed / Open Access
• [Journal Article] Separation and quantification of 2-acyl-1-lysophospholipids and 1-acyl-2-lysophospholipids in biological samples by LC-MS/MS. (2014)
  • Author(s): Okudaira M, Inoue A, Shuto A, Nakanaga K, Kano K, Makide K, Saigusa D, Tomioka Y, Aoki J.
  • Journal Title: J. Lipid Res. Volume: 55 Issue: 10 Pages: 2178-2192
  • DOI: 10.1194/jlr.d048439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ホスホリパーゼAの細胞内基質の同定 (2015)
  • Author(s): 川名裕己、中永景太、青木淳賢
  • Organizer: BMB2015
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-12-01