Challenge for the development of vaccine without the production of IgE

• Project/Area Number: 26670077
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Osaka University
• Principal Investigator: Yoshioka Yasuo 大阪大学, 微生物病研究所, 特任准教授(常勤) (00392308)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Takeharu 大阪大学, 産業科学研究所, 教授 (20311350) ; ARAI Yoshiyuki 大阪大学, 産業科学研究所, 助教 (20444515)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: IgE / ワクチン / 免疫 / ナノマテリアル / 免疫応答

Outline of Final Research Achievements

Here we tried to clarify the relationship between the dose of cutaneous exposure to antigen and the susceptibility to the IgE-mediated allergic response in relation to IgG production. NC/Nga mice were epicutaneously exposed to different doses of antigen to induce atopic dermatitis like skin lesions. We then evaluated the skin lesions, the induction of antigen-specific immune responses, and the susceptibility to anaphylaxis. Dose-dependent exacerbation of atopic dermatitis like skin lesions and antigen-specific IgG and IgE production were observed. However, mice exposed to relatively low doses of antigen showed hypersusceptibility to antigen specific anaphylaxis. We also showed that adoptive transfer of antigen specific IgG rescued mice from the hypersusceptibility seen in those exposed to low doses of antigen.

Research Products

• [Journal Article] High-dose cutaneous exposure to mite allergen induces IgG-mediated protection against anaphylaxis. (2015)
  • Author(s): Hirai T, Yoshioka Y, Takahashi H, Handa T, Izumi N, Mori T, Uemura E, Nishijima N, Sagami KI, Yamaguchi M, Eto SI, Nagano K, Kamada H, Tsunoda SI, Ishii KJ, Higashisaka K, Tsutsumi Y.
  • Journal Title: Clin Exp Allergy Volume: Feb 19 Issue: 7 Pages: 1-34
  • DOI: 10.1111/cea.12722
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cutaneous exposure to agglomerates of silica nanoparticles and allergen results in IgE-biased immune response and increased sensitivity to anaphylaxis in mice. (2015)
  • Author(s): Hirai T, Yoshioka Y, Takahashi H, Ichihashi K, Udaka A, Mori T, Nishijima N, Yoshida T, Nagano K, Kamada H, Tsunoda S, Takagi T, Ishii KJ, Nabeshi H, Yoshikawa T, Higashisaka K, Tsutsumi Y.
  • Journal Title: Part Fibre Toxicol. Volume: 12 Issue: 1 Pages: 1-14
  • DOI: 10.1186/s12989-015-0095-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant