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Development of a fluorescence correlation spectroscopy for rotational diffusion measurements

Research Project

Project/Area Number 26670086
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Terada Sumio  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022)

Co-Investigator(Kenkyū-buntansha) 川岸 将彦  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60323606)
齊藤 健太  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60374659)
Co-Investigator(Renkei-kenkyūsha) TANII Takashi  早稲田大学, 理工学術院, 教授 (20339708)
KONDO Takao  名古屋大学, 理学研究科, 教授 (10124223)
JIN Takashi  理化学研究所, 生命システム研究センター, チームリーダー (80206367)
Research Collaborator TANI Tomomi  米国ウッズホール海洋生物学研究所, 研究員
KINJO Masataka  北海道大学, 先端生命, 教授
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords顕微鏡技術 / 生体分子間相互作用ネットワーク / 蛍光相関分光法
Outline of Final Research Achievements

As rotational properties sensitively reflect molecular interactions and intracellular environment, polarization-dependent fluorescence correlation spectroscopy can be a promising method to analyse various cell biological events. We constructed both new polarization-dependent fluorescence correlation spectroscopy and an analysis program for this purpose. In addition, we could develop new methods to label proteins of interests for our analysis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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