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Polarized fluorescence microscopy analysis of nuclear lamina

Research Project

Project/Area Number 26670087
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Sato Keisuke  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60644044)

Co-Investigator(Kenkyū-buntansha) 寺田 純雄  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022)
川岸 将彦  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60323606)
齊藤 健太  東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60374659)
Research Collaborator TANI Tomomi  米国ウッズホール海洋学研究所, 研究員
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsラミン / 蛍光偏光 / 細胞分裂 / 細胞骨格 / 中間径フィラメント
Outline of Final Research Achievements

To analyze the structure of Lamin filaments, which is the main constituent of nuclear lamina of eukaryotic cells, human Lamin A and Lamin B1 proteins were constrainedly tagged with EGFP. We tried to replace endogenous Lamin genes of HeLa cells with cDNA of these proteins, and partially succeeded.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2015 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Int'l Joint Research] Marine Biological Laboratory(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ウッズホール海洋研究所(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210.2015

    • Author(s)
      Keisuke Sato, Peristera Roboti, Alexander A Mironov, Martin Lowe
    • Journal Title

      Mol. Biol. Cell

      Volume: 26 Issue: 3 Pages: 537-553

    • DOI

      10.1091/mbc.e14-10-1450

    • Related Report
      2014 Research-status Report
    • Peer Reviewed

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

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