Polarized fluorescence microscopy analysis of nuclear lamina

Research Project

Project/Area Number	26670087
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	General anatomy (including histology/embryology)
Research Institution	Tokyo Medical and Dental University
Principal Investigator	Sato Keisuke 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60644044)
Co-Investigator(Kenkyū-buntansha)	寺田純雄東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00262022) 川岸将彦東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60323606) 齊藤健太東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60374659)
Research Collaborator	TANI Tomomi 米国ウッズホール海洋学研究所, 研究員
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	ラミン / 蛍光偏光 / 細胞分裂 / 細胞骨格 / 中間径フィラメント
Outline of Final Research Achievements	To analyze the structure of Lamin filaments, which is the main constituent of nuclear lamina of eukaryotic cells, human Lamin A and Lamin B1 proteins were constrainedly tagged with EGFP. We tried to replace endogenous Lamin genes of HeLa cells with cDNA of these proteins, and partially succeeded.