| Project/Area Number |
26670096
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Tomoyuki 関西医科大学, 医学部, 教授 (20362527)
AOKI Hiroki 久留米大学, 付置研究所, 教授 (60322244)
MATSUSAKI Michiya 大阪大学, 工学(系)研究科, 助教 (00419467)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 血管 / 内弾性板 / 内皮細胞 / 動脈硬化 / 動脈瘤 |
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| Outline of Final Research Achievements |
The internal elastic lamina in the artery is located between the endothelium and the layers of smooth muscle cells. The internal elastic lamina maintains elasticity of the vessel and functions as a barrier to protect diffusion of various materials. Disrupt of the internal elastic lamina causes atherosclerosis and aneurysm of the artery. However, the mechanism of the internal elastic lamina formation has not been fully understood. In this study we hypothesized that a factor derived from the vascular endothelium promotes formation of the internal elastic lamina by interacting with smooth muscle cells. Using a hierarchical cell manipulation technique, we fabricated vascular smooth muscle cells into three-dimensional cellular multilayers and then a single layer of endothelial cells were covered into them. We further searched for a factor that promotes elastic fiber formation.
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