Live-imaging analysis of vesicle trafficking dynamics in isolated skeletal muscle fiber
Project/Area Number |
26670099
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General physiology
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Research Institution | Tohoku University |
Principal Investigator |
Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | 細胞内小胞輸送 / ライブイメージング / 骨格筋 / GLUT4 / イメージング / 細胞内小胞輸送系 / 量子ドット |
Outline of Final Research Achievements |
This study attempted to develop a novel approach for quantifying intracellular vesicle trafficking dynamics utilizing Quantum-dot-based single molecule imaging in a isolated skeletal muscle fiber from mouse. Intracellular dynamics of GLUT4, an insulin-responsive glucose transporter, as well as of transferrin receptor (TfR) were successfully quantified with this novel approach. In addition, the superresolution G-STED microscopy was employed to observe intracellular vesicles and organelles containing GLUT4 and/or TfR.
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Report
(3 results)
Research Products
(21 results)
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[Presentation] The Human Satellite Cells can Form the Myotube Even in the Atrophic Fatty Muscle of Rotator Cuff Tear2016
Author(s)
Koide M, Hagiwara Y, Ando A, Yabe Y, Kanazawa K, Sekiguchi T, Itaya N, Yoshida S, Itoi E, Kanzaki M, Hatakeyama H, Tsuchiya M
Organizer
The Orthopaedic Research Society 2016 Annual Meeting
Place of Presentation
Orlando, FL, USA
Year and Date
2016-03-05
Related Report
Int'l Joint Research
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