Live-imaging analysis of vesicle trafficking dynamics in isolated skeletal muscle fiber

• Project/Area Number: 26670099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tohoku University
• Principal Investigator: Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞内小胞輸送 / ライブイメージング / 骨格筋 / GLUT4 / イメージング / 細胞内小胞輸送系 / 量子ドット

Outline of Final Research Achievements

This study attempted to develop a novel approach for quantifying intracellular vesicle trafficking dynamics utilizing Quantum-dot-based single molecule imaging in a isolated skeletal muscle fiber from mouse. Intracellular dynamics of GLUT4, an insulin-responsive glucose transporter, as well as of transferrin receptor (TfR) were successfully quantified with this novel approach. In addition, the superresolution G-STED microscopy was employed to observe intracellular vesicles and organelles containing GLUT4 and/or TfR.

Research Products

• [Int'l Joint Research] Laval University Medical Center(Canada)
• [Int'l Joint Research] Francis Crick Institute(英国)
• [Int'l Joint Research] Aarhus University(Denmark)
• [Int'l Joint Research] Leibniz Center, Medicine & Biosciences(Germany)
• [Journal Article] 電気パルスを用いた収縮型培養筋細胞の創製 (2016)
  • Author(s): 神崎 展
  • Journal Title: 機械の研究 Volume: 68 (1) Pages: 56-58
• [Journal Article] Involvement of IL-1 in the Maintenance of Masseter Muscle Activity and Glucose Homeostasis. (2015)
  • Author(s): Chiba K, Tsuchiya M, Koide M, Hagiwara Y, Sasaki K, Hattori Y, Watanabe M, Sugawara S, Kanzaki M, Endo Y.
  • Journal Title: PLoS One Volume: 10 (11):e0143635 Issue: 11 Pages: e0143635-e0143635
  • DOI: 10.1371/journal.pone.0143635
  • Peer Reviewed / Open Access
• [Journal Article] Improvement of Cell Membrane Permeability Using a Cell-Solution Electrode for Generating Atmospheric-Pressure Plasma (2015)
  • Author(s): T. Kaneko, S. Sasaki, Y. Hokari, S. Horiuchi, R. Honda, and M. Kanzaki
  • Journal Title: Biointerphases Volume: 10 Issue: 2 Pages: 029521-029521
  • DOI: 10.1116/1.4921278
  • Peer Reviewed / Open Access
• [Journal Article] 脂質ラフト形成において細胞内外が共役するしくみ (2015)
  • Author(s): 神崎 展
  • Journal Title: 実験医学 Volume: 33(11) Pages: 1759-1760
• [Presentation] The Human Satellite Cells can Form the Myotube Even in the Atrophic Fatty Muscle of Rotator Cuff Tear (2016)
  • Author(s): Koide M, Hagiwara Y, Ando A, Yabe Y, Kanazawa K, Sekiguchi T, Itaya N, Yoshida S, Itoi E, Kanzaki M, Hatakeyama H, Tsuchiya M
  • Organizer: The Orthopaedic Research Society 2016 Annual Meeting
  • Place of Presentation: Orlando, FL, USA
  • Year and Date: 2016-03-05
  • Int'l Joint Research
• [Presentation] マウス走行運動モデルと電気パルス強収縮モデルを用いた収縮骨格筋の生物学的応答の解析―GLUT4制御に関わる分子基盤について― (2016)
  • Author(s): 細谷 雅浩、堰合 茂智、畠山 裕康、神崎 展
  • Organizer: 第4回骨格筋生物学研究会
  • Place of Presentation: 松本大学，長野
  • Year and Date: 2016-03-04
• [Presentation] Roles of exercise-induced gene, SPARC, against sarcopenia: link between extracellular matrix and mitochondria. (2015)
  • Author(s): Malouane A, Carbonell A, Yoshioka M, Kanzaki M, Puymirat J, St-Amand J
  • Organizer: 8th Cachexia Conference
  • Place of Presentation: Paris, France
  • Year and Date: 2015-12-04
  • Int'l Joint Research
• [Presentation] Cellular Nanoscience and Exercise-induced Health Benefits (2015)
  • Author(s): Kanzaki M, Hatakeyama H
  • Organizer: International Conference on Fluid Dynamics 2015, "Advanced Physical Stimuli and Biological Responses"
  • Place of Presentation: Sendai, International Center
  • Year and Date: 2015-10-28
  • Int'l Joint Research / Invited
• [Presentation] AS160 vs Tbc1d1: Cooperative determination of insulin-responsive GLUT4 trafficking activity after exercise-mimetic stimuli (2015)
  • Author(s): Hatakayama H, Kanzaki M
  • Organizer: 51st Annual Meeting of the European Association for the Study of Diabetes
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2015-09-14
  • Int'l Joint Research
• [Presentation] Coordinated actions of AS160 and Tbc1d1 as a determinant of insulin sensitivity in GLUT4 trafficking (2015)
  • Author(s): Hatakayama H, Kanzaki M
  • Organizer: FASEB SRC "Glucose Transport: Gateway to Metabolic Systems Biology"
  • Place of Presentation: Big Sky, MT, USA
  • Year and Date: 2015-07-26
  • Int'l Joint Research
• [Presentation] Involvement of Mechanosensitive Transcriptional Network in Contraction-dependent Muscle Fiber Type Conversion Analyzed using the "in vitro Exercise Model" (2015)
  • Author(s): Hosoya M, Uda Y, Hatakeyama H, Kanzaki M
  • Organizer: Cell Symposia "Exercise Metabolism"
  • Place of Presentation: Amsterdam, Netherland
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] 生細胞内における単一分子レベルの視覚化解析と分子動態生理学の創造 (2015)
  • Author(s): 神崎 展
  • Organizer: 日本動物細胞工学会2015年大会
  • Place of Presentation: 仙台・東北大学・片平さくらホール
  • Year and Date: 2015-07-09
  • Invited
• [Presentation] 廃用萎縮・脂肪変性したヒト筋組織の再生能力評価と脂肪変性抑制法の検討 (2015)
  • Author(s): 小出将志・土谷昌広・萩原嘉廣・神崎 展
  • Organizer: 第3回骨格筋生物学研究会
  • Place of Presentation: 仙台・東北大学星陵キャンパス
  • Year and Date: 2015-03-07 – 2015-03-09
• [Presentation] 運動機能維持におけるIL1の働き (2015)
  • Author(s): 土谷昌広・小出将志・萩原嘉廣・神崎 展
  • Organizer: 第3回骨格筋生物学研究会
  • Place of Presentation: 仙台・東北大学星陵キャンパス
  • Year and Date: 2015-03-07 – 2015-03-09
• [Remarks] 神崎研究室HP
  • URL: http://www.ecei.tohoku.ac.jp/kanzaki/index.html
• [Remarks] ResearchGate Makoto Kanzaki HP
  • URL: https://www.researchgate.net/profile/Makoto_Kanzaki/
• [Remarks] Research Gate HP
  • URL: https://www.researchgate.net/profile/Makoto_Kanzaki/