Molecular mechanism of brain histamine clearance

• Project/Area Number: 26670117
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Tohoku University
• Principal Investigator: YANAI Kazuhiko 東北大学, 医学(系)研究科(研究院), 教授 (50192787)
• Co-Investigator(Kenkyū-buntansha): WATANABE Takehiko 東北大学, 大学院医学系研究科, 名誉教授 (70028356) ; YOSHIKAWA Takeo 東北大学, 大学院医学系研究科, 助教 (70506633)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: ヒスタミン / HNMT / PMAT / ノックアウトマウス

Outline of Final Research Achievements

Histamine plays an important role in brain functions such as sleep-wake cycle and stress response. We investigated the molecular mechanism of histamine clearance. We demonstrated that histamine N-methyltransferase, which inactivates histamine, was one of the essential factors for histamine clearance.

Research Products

• [Journal Article] Insufficient intake of L-Histidine reduces brain histamine and causes anxiety-like behaviors in male mice. (2015)
  • Author(s): Takeo Yoshikawa, Tadaho Nakamura, Testuro Shibakusa, Mayu Sugita, Fumito Naganuma, Tomomitsu Iida, Yamato Miura, Attayeb Mohsen, Ryuichi Harada, and Kazuhiko Yanai
  • Journal Title: The Journal of nutrition Volume: 144 Issue: 10 Pages: 1637-1641
  • DOI: 10.3945/jn.114.196105
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of Histamine H3 Receptor in Glucagon-Secreting αTC1.6 cells (2015)
  • Author(s): Tadaho Nakamura, Takeo Yoshikawa, Fumito Naganuma, Attyeb Mohsen, Tomomitsu iida, Yamato Miura, Akira Sugawara and Kazuhiko Yanai
  • Journal Title: FEBS Open Bio Volume: 5 Issue: 1 Pages: 36-41
  • DOI: 10.1016/j.fob.2014.12.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Predominant role of plasma membrane monoamine transporters in monoamine transport in 1321N1 cells, a human astrocytoma-derived cell line. (2014)
  • Author(s): Fumito Naganuma, Takeo Yoshikawa, Tadaho Nakamura, Tomomistu Iida, Ryuichi Harada, Attayeb S. Mohsen, Yamato Miura and Kazuhiko Yanai.
  • Journal Title: Journal of Neurochemistry Volume: 129 Issue: 4 Pages: 591-601
  • DOI: 10.1111/jnc.12665
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mechanism of the histamine H3 receptor-mediated increase in exploratory locomotor activity and anxiety-like behaviours in mice (2014)
  • Author(s): Mohsen A, Yoshikawa T, Miura Y, Nakamura T, Naganuma F, Shibuya K, Iida T, Harada R, Okamura N, Watanabe T, Yanai K.
  • Journal Title: Neuropharmacology Volume: 81 Pages: 188-94
  • DOI: 10.1016/j.neuropharm.2014.02.003
  • Peer Reviewed / Open Access
• [Presentation] HistamineN-methyltransferaseノックアウトマウスの解析 (2015)
  • Author(s): 長沼史登、吉川雄朗、谷内一彦他
  • Organizer: 第８８回日本薬理学会
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Year and Date: 2015-03-18
• [Presentation] マウスにおける低親和性トランスポーターの輸送能解析 (2014)
  • Author(s): 三浦大和、吉川雄朗、谷内一彦ほか
  • Organizer: 第18回日本ヒスタミン学会
  • Place of Presentation: 都ホテルニューアルカイック（尼崎）
  • Year and Date: 2014-10-10
• [Remarks] 機能薬理学分野HP
  • URL: http://www.miec.umin.jp/cgi-bin/gaiyo.cgi
• [Remarks] 医学系研究科機能薬理学分野
  • URL: http://www.med.tohoku.ac.jp/org/medical/12/index.html
• [Remarks] サイクロトロン・ラジオアイソトープセンター
  • URL: http://www.cyric.tohoku.ac.jp/index_j.html