The roles of endogenous retrotransposition in somatic cell reprogramming

• Project/Area Number: 26670137
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: Yamamoto Takuya 京都大学, iPS細胞研究所, 助教 (60546993)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ゲノム / 発生・分化 / 発生･分化

Outline of Final Research Achievements

Recent studies have demonstrated that genomic changes driven by retrotransposition could occur frequently in early embryonic development and neural progenitor cells. In this study, to elucidate contributions of retrotransposition to somatic cell reprogramming, we have identified insertion sites of retrotransposons at the genome-wide scale in human iPS cells by using Retrotransposon Capture Sequencing (RC-Seq). In addition, we found the genomic insertion sites of retrotransposons that were newly acquired during reprogramming, and examined the relationship between the insertion sites and somatic cell reprogramming.