Molecular mechanisms of regulation of intestinal immunity by intestinal epithelial cells
Project/Area Number |
26670142
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Kobe University |
Principal Investigator |
Matozaki Takasi 神戸大学, 医学(系)研究科(研究院), 教授 (80252782)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 腸上皮細胞 / 炎症 / 生体分子 / 腸内細菌 / シグナル伝達 |
Outline of Final Research Achievements |
SAP-1 is a receptor-type protein tyrosine phosphatase that is localized specifically at microvilli of the brush border in intestinal epithelial cells. I previously found that SAP-1 ablation results in exacerbation of spontaneous colitis in Il10 knockout mice. In this study, I showed that tyrosine phosphorylation of CEACAM, a dephosphorylation substrate for SAP-1, promotes the activation of downstream molecules, thereby inducing production of chemokines such as IL-8. In addition, I found that SAP-1 specifically interacts with CEACAM and that this interaction is mediated via the ectodomains of both proteins. Thus, SAP-1 in intestinal epithelial cells is likely to regulate the intestinal immunity by controlling the tyrosine phosphorylation level of CEACAM.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Protein tyrosine phosphatase SAP-1 protects against colitis through regulation of CEACAM20 in the intestinal epithelium2015
Author(s)
Y Murata, T Kotani, Y Supriatna, Y Kitamura, S Imada, K Kawahara, M Nishio, EW Daniwijaya, H Sadakata, S Kusakari, M Mori, Y Kanazawa, Y Saito, K Okawa, M Takeda-Morishita, H Okazawa, H Ohnishi, T Azuma, A Suzuki, T Matozaki
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Journal Title
Proc Natl Acad Sci U S A
Volume: 112
Issue: 31
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Dendritic cell SIRPα regulates homeostasis of dendritic cells in lymphoid organs2015
Author(s)
Washio K, Kotani T, Saito Y, Respatika D, Murata Y, Kaneko Y, Okazawa H, Ohnishi H, Fukunaga A, Nishigori C, Matozaki T.
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Journal Title
Genes Cells
Volume: -
Issue: 6
Pages: 00-00
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Shp2 in forebrain neurons regulates synaptic plasticity, locomotion, and memory formation in mice2015
Author(s)
Kusakari S, Saitow F, Ago Y, Shibasaki K, Sato-Hashimoto, M., Matsuzaki, Y., Kotani T, Murata Y, Hirai H, Matsuda T, Suzuki H, Matozaki T, Ohnishi H
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Journal Title
Mol. Cell. Biol.
Volume: 35
Issue: 9
Pages: 1557-1572
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Protection against high-fat diet-induced obesity in Helz2-deficient male mice due to enhanced expression of hepatic leptin receptor2014
Author(s)
Yoshino S, Satoh T, Yamada M, Hashimoto K, Tomaru T, Katano-Toki A, Kakizaki S, Okada S, Shimizu H, Ozawa A, Tuchiya T, Ikota H, Nakazato Y, Mori M, Matozaki T, Sasaki T, Kitamura T, Mori M.
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Journal Title
Endocrinology.
Volume: 155
Issue: 9
Pages: 3459-3472
DOI
Related Report
Peer Reviewed
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