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Development of high-throughput detection method of tRNA modifications

Research Project

Project/Area Number 26670284
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionKumamoto University

Principal Investigator

Tomizawa Kazuhito  熊本大学, 大学院生命科学研究部(医), 教授 (40274287)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords糖尿病 / ミトコンドリア / tRNA / 化学修飾 / 定量PCR / 疾患マーカー / オミックス / 質量分析 / 癌 / 臨床研究
Outline of Final Research Achievements

Modification of tRNAs is a critical factor underlying the development of diabetes, and the measurements of the modification level could be used as a clinical biomarker for the diagnosis of diabetes. However, there is no convenient method to detect tRNA modifications. Current methods, such as mass spectrometry and primer extension method, are not ideal for the easily and reliably detection of such modifications in clinical samples. Here, I present a new method (qPCR-MtR) that overcomes the disadvantages of the current methods and can detect and quantify the modification of tRNAs in multiple samples. The method was sensitive enough to measure 2-methlthio (ms2) modification levels of tRNALys(UUU) in total RNA sample isolated from human peripheral blood specimens and revealed that ms2-modification levels in tRNALys(UUU) were lower in individuals carrying higher-risk SNP of cdkal1.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Remarks (2 results)

  • [Int'l Joint Research] エクセター大学医学部(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Measurement of 2-methylthio Modifications in Mitochondrial Transfer RNAs by Reverse-transcription Quantitative PCR.2016

    • Author(s)
      Wei, F.-Y., and Tomizawa, K.
    • Journal Title

      Bio-protocol

      Volume: 6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans.2015

    • Author(s)
      Wei FY, Zhou B, Suzuki T, Miyata K, Ujihara Y, Horiguchi H, Takahashi N, Xie P, Michiue H, Fujimura A, Kaitsuka T, Matsui H, Koga Y, Mohri S, Suzuki T, Oike Y, Tomizawa K.
    • Journal Title

      Cell Metab

      Volume: 21 Issue: 3 Pages: 413-6

    • DOI

      10.1016/j.cmet.2015.01.019

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A cautionary tale: the non-causal association between type 2 diabetes risk SNP, rs7756992, and levels of non-coding RNA, CDKAL1-v1.2015

    • Author(s)
      Locke JM, Wei FY, Tomizawa K, Weedon MN, Harries LW.
    • Journal Title

      Diabetologia

      Volume: 58 Issue: 4 Pages: 745-748

    • DOI

      10.1007/s00125-015-3508-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Remarks] 熊本大学大学院生命科学研究部・分子生理学分野ホームページ

    • URL

      http://kumamoto-physiology.jp

    • Related Report
      2015 Annual Research Report
  • [Remarks] 熊本大学大学院生命科学研究部・分子生理学分野

    • URL

      http://kumamoto-physiology.jp

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-01-31  

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