Disease modeling for SCVTdP by iPS cells

• Project/Area Number: 26670408
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Keio University
• Principal Investigator: Yuasa Shinsuke 慶應義塾大学, 医学部, 講師 (90398628)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: iPS細胞 / 心筋細胞 / 不整脈

Outline of Final Research Achievements

We generated iPS cells from patients with short coupled variant TdP (SCVTdP). Cardiomyocytes were differentated from control-iPS cells and SCVTdP-iPS cells. Then we performed the electrophysiological analysis using iPS cell-derived cardiomyocytes. We identified the specific phenotype in SCVTdP-iPS cell-derived cardiomyocytes.

Research Products

• [Journal Article] Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells. (2016)
  • Author(s): Tohyama S, Fujita J, Hishiki T, Matsuura T, Hattori F, Ohno R, Kanazawa H, Seki T, Nakajima K, Kishino Y, Okada M, Hirano A, Kuroda T, Yasuda S, Sato Y, Yuasa S, Sano M, Suematsu M, Fukuda K.
  • Journal Title: Cell Metab Volume: 23 Issue: 4 Pages: 663-74
  • DOI: 10.1016/j.cmet.2016.03.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Novel Wire-Assisted Technique for Closing Large Atrial Septal Defects: New Concepts of Closure Mechanism. (2016)
  • Author(s): Kanazawa H, Kawamura A, Kimura M, Akita K, Yashima F, Arai T, Kawakami T, Hayashida K, Tsuruta H, Yuasa S, Itabashi Y, Murata M, Maekawa Y, Fukuda K.
  • Journal Title: JACC Cardiovasc Interv Volume: 9 Issue: 6 Pages: e59-e61
  • DOI: 10.1016/j.jcin.2015.12.016
  • Peer Reviewed
• [Journal Article] In-Stent Dissection Causes No Flow During Percutaneous Coronary Intervention. (2016)
  • Author(s): Yashima F, Yuasa S, Maekawa Y, Kimura M, Akita K, Yanagisawa R, Tanaka M, Hayashida K, Kawakami T, Kanazawa H, Fujita J, Fukuda K.
  • Journal Title: JACC Cardiovasc Interv. Volume: 9 Issue: 1 Pages: 9-9
  • DOI: 10.1016/j.jcin.2015.09.025
  • Peer Reviewed
• [Journal Article] "Moving left ventricular obstruction" due to stress cardiomyopathy in a patient with hypertrophic obstructive cardiomyopathy treated with percutaneous transluminal septal myocardial ablation. (2016)
  • Author(s): Akita K, Maekawa Y, Tsuruta H, Okuda S, Yanagisawa R, Kageyama T, Kawakami T, Kanazawa H, Hayashida K, Yuasa S, Murata M, Jinzaki M, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 202 Pages: 194-195
  • DOI: 10.1016/j.ijcard.2015.08.145
  • Peer Reviewed
• [Journal Article] Patient-Specific Induced Pluripotent Stem Cell Models: Characterization of iPS Cell-Derived Cardiomyocytes. (2016)
  • Author(s): Egashira T, Yuasa S, Tohyama S, Kuroda Y, Suzuki T, Seki T, Fukuda K.
  • Journal Title: Methods Mol Biol. Volume: 1353 Pages: 343-53
  • DOI: 10.1007/7651_2014_165
  • ISBN: 9781493930333, 9781493930340
  • Peer Reviewed
• [Journal Article] Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions. (2015)
  • Author(s): Kishino Y, Seki T, Yuasa S, Fujita J, Fukuda K.
  • Journal Title: J Vis Exp. Volume: 105 Issue: 105 Pages: 105-105
  • DOI: 10.3791/53225
  • Peer Reviewed
• [Journal Article] "Protruding Myocardium" as a Target for Percutaneous Transluminal Septal Myocardial Ablation in a Case of Hypertrophic Obstructive Cardiomyopathy. (2015)
  • Author(s): Maekawa Y, Akita K, Tsuruta H, Yashima F, Kimura M, Yamada Y, Kawakami T, Kanazawa H, Hayashida K, Yuasa S, Murata M, Jinzaki M, Fukuda K.
  • Journal Title: JACC Cardiovasc Interv. Volume: 8 Issue: 12 Pages: 201-202
  • DOI: 10.1016/j.jcin.2015.05.029
  • Peer Reviewed
• [Journal Article] Cardiovascular Disease Modeling Using Patient-Specific Induced Pluripotent Stem Cells. (2015)
  • Author(s): Tanaka A, Yuasa S, Node K, Fukuda K.
  • Journal Title: Int J Mol Sci. Volume: 16 Issue: 8 Pages: 18894-922
  • DOI: 10.3390/ijms160818894
  • Peer Reviewed
• [Journal Article] Analysis of cardiomyocyte movement in the developing murine heart. (2015)
  • Author(s): Hashimoto H, Yuasa S, Tabata H, Tohyama S, Seki T, Egashira T, Hayashiji N, Hattori F, Kusumoto D, Kunitomi A, Takei M, Kashimura S, Yozu G, Shimojima M, Motoda C, Muraoka N, Nakajima K, Sakaue-Sawano A, Miyawaki A, Fukuda K
  • Journal Title: Biochem Biophys Res Commun Volume: 464 Issue: 4 Pages: 1000-1007
  • DOI: 10.1016/j.bbrc.2015.07.036
  • Peer Reviewed / Open Access
• [Journal Article] The Role of Circadian Rhythms in Fatal Arrhythmias and the Potential Impact of Intervention for Sleep-Disordered Breathing. (2015)
  • Author(s): Aizawa Y, Kohno T, Yuasa S, Fukuda K.
  • Journal Title: Curr Pharm Des. Volume: 21 Pages: 3512-22
  • Peer Reviewed
• [Journal Article] G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy (2015)
  • Author(s): Hayashiji N, Yuasa S, Miyagoe-Suzuki Y, Hara M, Ito N, Hashimoto H, Kusumoto D, Seki T, Tohyama S, Kodaira M, Kunitomi A, Kashimura S, Takei M, Saito Y, Okata S, Egashira T, Endo J, Sasaoka T, Takeda S, Fukuda K
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6745-6745
  • DOI: 10.1038/ncomms7745
  • Peer Reviewed / Open Access
• [Journal Article] Lesion morphological classification by OCT to predict therapeutic efficacy after balloon pulmonary angioplasty in CTEPH. (2015)
  • Author(s): Inohara T, Kawakami T, Kataoka M, Yamamoto M, Kimura M, Kanazawa H, Yuasa S, Hayashida K, Maekawa Y, Fukuda K.
  • Journal Title: Lesion morphological classification by OCT to predict therapeutic efficacy after balloon pulmonary angioplasty in CTEPH. Volume: 197 Pages: 2015-2015
  • DOI: 10.1016/j.ijcard.2015.06.036
  • Peer Reviewed
• [Journal Article] Fractured mobile flap in pulmonary artery: One of possible mechanisms for residual or recurrent pulmonary hypertension after pulmonary endarterectomy. (2015)
  • Author(s): Arai T, Kawakami T, Kataoka M, Yuasa S, Maekawa Y, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 185 Pages: 129-130
  • DOI: 10.1016/j.ijcard.2015.02.054
  • Peer Reviewed
• [Journal Article] Improved renal function in a patient with hypertrophic obstructive cardiomyopathy after multidetector computed tomography-guided percutaneous transluminal septal myocardial ablation. (2015)
  • Author(s): Maekawa Y, Jinzaki M, Tsuruta H, Akita K, Yamada Y, Kawakami T, Hayashida K, Yuasa S, Murata M, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 181 Pages: 349-50
  • DOI: 10.1016/j.ijcard.2014.12.078
  • Peer Reviewed
• [Journal Article] High-Temperature Requirement A Serine Peptidase 1 Gene is Transcriptionally Regulated by Insertion/Deletion Nucleotides Located at the 3 Prime End of Age-Related Maculopathy Susceptibility 2 Gene in Patients with Age-Related Macular Degeneration. (2015)
  • Author(s): Iejima D, Itabashi T, Kawamura Y, Noda T, Yuasa S, Fukuda K, Oka C, Iwata T.
  • Journal Title: The Journal of Biological Chemistry Volume: 290 Issue: 5 Pages: 2784-2797
  • DOI: 10.1074/jbc.m114.593384
  • Peer Reviewed
• [Journal Article] Prognostic implications of optimal medical therapy in patients undergoing percutaneous coronary intervention for acute coronary syndrome in octogenarians. (2015)
  • Author(s): Anzai A, Maekawa Y, Kodaira M, Mogi S, Arai T, Kawakami T, Kanazawa H, Hayashida K, Yuasa S, Kawamura A, Fukuda K.
  • Journal Title: Heart Vessels. Volume: 30(2) Issue: 2 Pages: 186-92
  • DOI: 10.1007/s00380-014-0474-y
  • Peer Reviewed
• [Journal Article] Reprogramming suppresses premature senescence phenotypes of Werner syndrome cells and maintains chromosomal stability over long-term culture (2014)
  • Author(s): Shimamoto A, Kagawa H, Zensho K, Sera Y, Kazuki Y, Osaki M, Oshimura M, Ishigaki Y, Hmasaki K, Kodama Y, Yuasa S, Fukuda K, Hirashima K, Seimiya H, Koyama H, Shimizu T, Takemoto M, Yokote K, Goto M, Tahara H.
  • Journal Title: PLOS ONE Volume: 9 Issue: 11 Pages: 1-13
  • DOI: 10.1371/journal.pone.0112900
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ET-1 induces myofibrillar disarray and contractile vector in hypertrophic cardiomyopathy-iPS cell-derived cardiomyocytes (2014)
  • Author(s): Tanaka A, Yuasa S, Mearini G, Egashira T, Seki T, Kodaira M, Kusumoto D, Kuroda Y, Okata S, Suzuki T, Arimura T, Makino S, Kimura K, Kimura A, Furukawa T, Carrier L, Nobe K, Fukuda K
  • Journal Title: J Am Heart Assoc Volume: 3 Issue: 6 Pages: 1-25
  • DOI: 10.1161/jaha.114.001263
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A massive suspension culture system with metabolic purification for human pluripotent stem cell-derived cardiomyocytes. (2014)
  • Author(s): Hemmi N**, Tohyama S**, Nakajima K, Kanazawa H, Suzuki T, Hattori F, Seki T, Kishino Y, Hirano A, Okada M, Tabei R, Ohno R, Fujita C, Haruna T, Yuasa S, Sano M, Fujita J, Fukuda K.
  • Journal Title: Stem Cells Translational Medicine Volume: ３ Issue: 12 Pages: 1473-83
  • DOI: 10.5966/sctm.2014-0072
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Successful second attempt multidetector computed tomography-guided percutaneous transluminal septal myocardial ablation for an octogenarian with hypertrophic obstructive cardiomyopathy. (2014)
  • Author(s): Maekawa Y, Jinzaki M, Anzai A, Tsuruta H, Matsumura K, Yamada Y, Tabei R, Kawakami T, Hayashida K, Yuasa S, Murata M, Suzuki M, Kuribayashi S, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 176(3) Issue: 3 Pages: e131-e132
  • DOI: 10.1016/j.ijcard.2014.07.211
  • Peer Reviewed
• [Journal Article] "Phantom vessel" running parallel to the culprit artery in a case of acute myocardial infarction. (2014)
  • Author(s): Anzai A, Yuasa S, Kanazawa H, Kodaira M, Arai T, Kawakami T, Hayashida K, Maekawa Y, Kawamura A, Fukuda K.
  • Journal Title: JACC Cardiovasc Interv Volume: 7(6） Issue: 6 Pages: e51-e52
  • DOI: 10.1016/j.jcin.2013.08.020
  • Peer Reviewed
• [Journal Article] Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions. (2014)
  • Author(s): Kishino Y, Seki T, Fujita J, Yuasa S, Tohyama S, Kunitomi A, Tabei R, Nakajima K, Okada M, Hirano A, Kanazawa H, Fukuda K.
  • Journal Title: PLoS One. Volume: 9(5) Issue: 5 Pages: e97397-e97397
  • DOI: 10.1371/journal.pone.0097397
  • Peer Reviewed / Open Access
• [Journal Article] Thoracic impedance as a therapeutic marker of acute decompensated heart failure (2014)
  • Author(s): Aizawa Y, Takatsuki S, Kashimura S, Katsumata Y, Nishiyama T, Kimura T, Nishiyama N, Tanimoto Y, Tanimoto K, Fukuda Y, Sato T, Ogawa S, Fukuda K
  • Journal Title: Int J Cardiol Volume: 174 Issue: 2 Pages: 840-2
  • DOI: 10.1016/j.ijcard.2014.04.032
  • Peer Reviewed
• [Journal Article] Utility of the reverse wire technique in multidetector computed tomography-guided percutaneous transluminal septal myocardial ablation. (2014)
  • Author(s): Maekawa Y, Jinzaki M, Anzai A, Matsumura K, Tsuruta H, Kawakami T, Hayashida K, Yuasa S, Murata M, Suzuki M, Kuribayashi S, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 173(3) Issue: 3 Pages: e33-e34
  • DOI: 10.1016/j.ijcard.2014.03.057
  • Peer Reviewed
• [Journal Article] Time-lapse imaging of cell cycle dynamics during development in living cardiomyocyte. (2014)
  • Author(s): Hashimoto H, Yuasa S, Tabata H, Tohyama S, Hayashiji N, Hattori F, Muraoka N, Egashira T, Okata S, Yae K, Seki T, Nishiyama T, Nakajima K, Sakaue-Sawano A, Miyawaki A and Fukuda K.
  • Journal Title: J.Mol. Cell. Cardiol. Volume: S0022-2828(14) Pages: 97-2
  • DOI: 10.1016/j.yjmcc.2014.03.020
  • Peer Reviewed