Epigenetic Regulation of hematopoietic stem cells by O-GlcNAc transferase
Project/Area Number |
26670469
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Yokohama City University (2015) Keio University (2014) |
Principal Investigator |
NAKAJIMA Hideaki 横浜市立大学, 医学(系)研究科(研究院), 教授 (30217723)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | O結合型β-N-アセチルグルコサミン転移酵素 / 糖鎖修飾 / 造血幹細胞 / 造血前駆細胞 / 胎仔肝 / 胎児肝 / O-GlcNAc / Ogt / 胎仔肝造血 |
Outline of Final Research Achievements |
O-linked-β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) is a critical enzyme that catalyzes O-GlcNAcylation of Ser/Thr residues of various proteins. This study examined the physiological role of OGT in hematopoietic system using OGT conditional knockout mice. OGT-deficient fetal liver cells were severely decreased in number and lost colony-forming activity in methylcellulose culture. Moreover, self-renewal and long-term repopulating capacity of OGT-deficient fetal liver hematopoietic stem cells were severely impaired in transplantation assays. These results indicated that OGT played a critical role in fetal liver hematopoiesis.
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Report
(3 results)
Research Products
(1 results)