Epigenetic Regulation of hematopoietic stem cells by O-GlcNAc transferase

• Project/Area Number: 26670469
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Yokohama City University (2015); Keio University (2014)
• Principal Investigator: NAKAJIMA Hideaki 横浜市立大学, 医学(系)研究科(研究院), 教授 (30217723)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: O結合型β-N-アセチルグルコサミン転移酵素 / 糖鎖修飾 / 造血幹細胞 / 造血前駆細胞 / 胎仔肝 / 胎児肝 / O-GlcNAc / Ogt / 胎仔肝造血

Outline of Final Research Achievements

O-linked-β-N-acetylglucosamine (O-GlcNAc) transferase （OGT） is a critical enzyme that catalyzes O-GlcNAcylation of Ser/Thr residues of various proteins. This study examined the physiological role of OGT in hematopoietic system using OGT conditional knockout mice. OGT-deficient fetal liver cells were severely decreased in number and lost colony-forming activity in methylcellulose culture. Moreover, self-renewal and long-term repopulating capacity of OGT-deficient fetal liver hematopoietic stem cells were severely impaired in transplantation assays. These results indicated that OGT played a critical role in fetal liver hematopoiesis.

Research Products

• [Presentation] A CRITICAL ROLE FOR O-GLCNACYLATION IN MURINE FETAL LIVER AND ADULT HEMATOPOIESIS (2015)
  • Author(s): Shunsuke Soma, Yumi Fukuchi, Shinichiro Okamoto, Hideaki Nakajima
  • Organizer: 20th Congress of European Hematology Association
  • Place of Presentation: ウィーン、オーストリア
  • Year and Date: 2015-06-11
  • Int'l Joint Research