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Identification of lncRNAs during B cell lineage commitment

Research Project

Project/Area Number 26670472
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Tomokatsu Ikawa  国立研究開発法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (60450392)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsB細胞分化 / 長鎖ノンコーディングRNA / 造血幹細胞 / 運命決定 / エピジェネティクス
Outline of Final Research Achievements

B lymphocytes are generated from pluripotent hematopoietic stem cells (HSCs) through a successive series of lineage restriction processes. Although many transcription factors and epigenetic modifiers were found to regulate the B cell lineage commitment, the exact mechanisms were yet to be determined. We have recently established an ideal system that can be used to examine gene regulatory networks during lymphoid lineage specification from HSCs. In this study, we looked for novel long non coding (lnc) RNAs that could regulate B cell fate determination using this system. We have succeeded in identifying the lncRNAs that may be involved in the specification and commitment of HSCs toward the B cell lineage.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 Other

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 8 results,  Acknowledgement Compliant: 6 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 7 results) Remarks (1 results)

  • [Journal Article] Role of Polycomb RYBP in maintaining the B-1 to B-2 B-cell lineage switch in adult hematopoiesis2015

    • Author(s)
      Cales C, Pavon L, Starowicz K, Perrez C, Bravo M, Ikawa T, Koseki H, Vidal M
    • Journal Title

      Mol Cell Biol.

      Volume: 36 Issue: 6 Pages: 900-912

    • DOI

      10.1128/mcb.00869-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Induced developmental arrest of early hematopoietic progenitors leads to the generation of leukocyte stem cells2015

    • Author(s)
      Ikawa T, Masuda K, Huijskens MJAJ, Satoh R, Kakugawa K, Agata Y, Miyai T, Germeraad WTV, Katsura Y, Kawamoto H
    • Journal Title

      Stem Cell Reports

      Volume: 5 Pages: 716-727

    • NAID

      120005745636

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability.2015

    • Author(s)
      Takeshi Inoue, Masahiro Morita, Atsushi Hijikata, Yoko Fukuda-Yuzawa, Shungo Adachi, Kyoichi Isono, Tomokatsu Ikawa, Hiroshi Kawamoto, Haruhiko Koseki, Tohru Natsume, Taro Fukao, Osamu Ohara, Tadashi Yamamoto, Tomohiro Kurosaki
    • Journal Title

      Journal of Experimental Medicine

      Volume: 212 Issue: 9 Pages: 1465-1479

    • DOI

      10.1084/jem.20150384

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] BTB-ZF Protein Znf131 Regulates Cell Growth of Developing and Mature T Cells.2015

    • Author(s)
      Iguchi T, Aoki K, Ikawa T, Taoka M, Taya C, Yoshitani H, Toma-Hirano M, Koiwai O, Isobe T, Kawamoto H, Masai H, Miyatake S.
    • Journal Title

      The Journal of Immunology

      Volume: 195 Issue: 3 Pages: 982-93

    • DOI

      10.4049/jimmunol.1500602

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Gene regulation. Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells2015

    • Author(s)
      Arner E, Daub CO, Vitting-Seerup K, Andersson R, Lilje B, Drabløs F, Lennartsson A, Rönnerblad M, Hrydziuszko O, Vitezic M, Freeman TC, Alhendi AM, Arner P, Axton R, Baillie JK, Beckhouse A, Bodega B, Briggs J, Brombacher F, Davis M, Detmar M, Ehrlund A, Endoh M, Eslami A, Fagiolini M, et al.
    • Journal Title

      Science

      Volume: 347 Issue: 6225 Pages: 1010-1014

    • DOI

      10.1126/science.1259418

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The transcription repressors Bach2 and Bach1 promote B cell development by repressing myeloid program.2014

    • Author(s)
      Itoh-Nakadai A, Hikota R, Muto A, Kometani K, Matsui-Watanabe M, Sato Y, Kobayashi M, Nakamura A, Miura Y, Yano Y, Tashiro S, Sun J, Ikawa T, Ochiai K, Kurosaki T, and Igarashi K.
    • Journal Title

      Nature Immunol.

      Volume: 15 Issue: 12 Pages: 1171-1180

    • DOI

      10.1038/ni.3024

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Zinc transporter SLC39A10/ZIP10 controls humoral immunity by modulating B-cell receptor signal strength.2014

    • Author(s)
      Hojyo S., T. Miyai, H. Fujishiro, M. Kawamura, T. Yasuda, A. Hijikata, B-H Bin, T. Irie, J. Tanaka, T. Atsumi, M. Murakami, M. Nakayama, O. Ohara, S. Himeno, H. Yoshida, H. Koseki, T. Ikawa, K. Mishima, and T. Fukada.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 111 Issue: 32 Pages: 11786-11791

    • DOI

      10.1073/pnas.1323557111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Zinc transporter SLC39A10/ZIP10 facilitates antiapoptotic signaling during early B-cell development2014

    • Author(s)
      Miyai T, Hojyo S, Ikawa T, Kawamura M, Irie T, Ogura H, Hijikata A, Bin BH, Yasuda T, Kitamura H, Nakayama M, Ohara O, Yoshida H, Koseki H, Mishima K, Fukada T
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 111 Issue: 32 Pages: 11780-11785

    • DOI

      10.1073/pnas.1323549111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic and epigenetic control of early lymphocyte development2014

    • Author(s)
      Ikawa T
    • Journal Title

      Curr Top Microbiol Immunol.

      Volume: 381 Pages: 1-20

    • DOI

      10.1007/82_2014_370

    • ISBN
      9783319073941, 9783319073958
    • Related Report
      2014 Research-status Report
    • Open Access / Acknowledgement Compliant
  • [Journal Article] The epigenetic regulator Uhrf1 facilitates proliferation and maturation of colonic regulatory T cells2014

    • Author(s)
      Yuuki Obata*, Yukihiro Furusawa*, Takaho A. Endo, Jafar Sharif, Daisuke Takahashi, Koji Atarashi, Manabu Nakayama, Satoshi Onawa, Yumiko Fujimura, Masumi Takahashi, Tomokatsu Ikawa, Takeshi Otsubo, Yuki I. Kawamura, Taeko Dohi, Shoji Tajima, Osamu Ohara, Kenya Honda, Shohei Hori, Hiroshi Ohno, Haruhiko Koseki and Koji Hase. 【* : 筆頭筆者】
    • Journal Title

      Nature Immunology

      Volume: 15 Issue: 6 Pages: 571-579

    • DOI

      10.1038/ni.2886

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Identification of gene regulatory networks during hematopoietic stem cell differentiation by the single-cell analysis2016

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      Single cell Workshop2016
    • Place of Presentation
      吹田市 理研QBiC
    • Year and Date
      2016-02-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gene regulatory networks that orchestrate T and B lymphocyte development2016

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      Liaison Laboratory seminar
    • Place of Presentation
      熊本市 熊本大学
    • Year and Date
      2016-02-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Transcriptional networks that control B cell fate determination2015

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      BMB2015
    • Place of Presentation
      神戸市 神戸国際展示場
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Role of IRS-1 in early B cell development2015

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      日本免疫学会2015
    • Place of Presentation
      札幌市 札幌コンベンションセンター
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Epigenetic maintenance of T cell fate by Polycomb group proteins2015

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      内藤カンファレンス
    • Place of Presentation
      札幌市 シャトレーゼ ガトーキングダムサッポロ
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] B細胞系列への運命決定過程における転写制御ネットワークの解明2015

    • Author(s)
      宮井智浩、伊川友活
    • Organizer
      KTCC
    • Place of Presentation
      京都 京都大学芝蘭会館
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gene regulatory network orchestrating B cell fate specification2015

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      RIKEN EPIGENETICS in Kobe
    • Place of Presentation
      神戸市 理研 CDB
    • Year and Date
      2015-02-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Epigenetic maintenance of T cell identity by Polycomb-mediated suppression of Pax52014

    • Author(s)
      Tomokatsu Ikawa
    • Organizer
      日本免疫学会
    • Place of Presentation
      京都市 国立京都国際会館
    • Year and Date
      2014-12-11
    • Related Report
      2014 Research-status Report
  • [Presentation] T細胞系列への運命決定とエピジェネティクス2014

    • Author(s)
      伊川 友活
    • Organizer
      LEGENDセミナー
    • Place of Presentation
      京都市 ホテルグランヴィア京都
    • Year and Date
      2014-12-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Maintenance of T cell identity by Polycomb group proteins2014

    • Author(s)
      Ikawa T
    • Organizer
      France-Japan Immunology meeting
    • Place of Presentation
      L'Oustau Calendal, Cassis, France
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Genetic and epigenetic control of early lymphocyte development2014

    • Author(s)
      Ikawa T
    • Organizer
      Max Planck Institute seminar
    • Place of Presentation
      Max Planck Institute, Freiburg, Germany
    • Year and Date
      2014-10-20
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Polycomb group proteins Ring1A/B are required for the maintenance of T cell fate2014

    • Author(s)
      Ikawa T
    • Organizer
      CSHL meeting
    • Place of Presentation
      Cold Spring Harbor Laboratory, Cold Spring Harbor, USA
    • Year and Date
      2014-04-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞分化の研究に魅せられて2014

    • Author(s)
      伊川 友活
    • Organizer
      新学術領域 「細胞運命制御」 若手の会
    • Place of Presentation
      浜松市 浜名湖ロイヤルホテル
    • Year and Date
      2014-04-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 多能前駆細胞を無限に増幅させる方法を開発

    • URL

      http://www.riken.jp/pr/press/2015/20151023_1/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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