Role of CD26 molecule in the infection of MERS(Middle East respiratory syndrome) virus and development of new therapy
| Project/Area Number |
26670486
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Infectious disease medicine
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
MORIMOTO CHIKAO 順天堂大学, 医学(系)研究科(研究院), 客員教授 (30119028)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IWATA Satoshi 順天堂大学, 医学部, 非常勤講師 (00396871)
OHNUMA Kei 順天堂大学, 医学部, 准教授 (10396872)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | MERS感染症 / コロナウイルス / CD26/DPPⅣ / 重症呼吸器感染症 / ヒト化CD26抗体 / CD26単クローン抗体 / アポトーシス / エピトープ / (1) MERS感染症 / トランスジェニックマウス / ① MERS感染症 / ② コロナウイルス / CD26 / ④ 重症呼吸器感染症 / ⑤ ヒト化CD26抗体 / ⑦ CD26単クローン抗体 |
|---|
| Outline of Final Research Achievements |
We identified the domains of CD26 involved in the binding of Middle East respiratory syndrome coronavirus using distinct clones of anti-CD26 monoclonal antibodies (MAbs). One clone, named 2F9, almost completely inhibited viral entry. The humanized anti-CD26 MAb also significantly inhibited infection. These findings indicate that both 2F9 and humanized Mab are potential therapeutic agents for MERS infection.
MERS infects human bronchial epithelial Calu-3 cells as well as CD26 Jurkat T lymphocyte cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-3 cells. Infection results in profound apoptosis irrespective of its production of titers that are lower than those of SARS-CoV. Together, our results provide new insights into the dissemination and pathogenesis of MERS-CoV and may cause in immune defects since it infected in human CD26 T cells.
|
Report
(3 results)
Research Products
(30 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma.2014
Author(s)
Yamamoto J, Ohnuma K, Hatano R, Okamoto T, Komiya E, Yamazaki H, Iwata S, Dang NH, Aoe K, Kishimoto T, Yamada T, Morimoto C.
-
Journal Title
Br J Cancer.
Volume: Vol 110
Issue: 9
Pages: 2232-45
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
[Presentation] First -in- human phase I administration of YS110, a monoclonal antibody directed against the CD26 immunostimulatory molecule in advanced cancer patients2015
Author(s)
Angevin E, Isambert N,Trillet-Lenoir VN,You V, Alxandre J, Zalcman G, Viel P, Farace F, Valleix F, Podoll T, Kuramochi Y, Miyashita I, HosonoO, Dang NH, Ohnuma K, Yamada T, Kaneko Y, Morimoto C.
Organizer
The 2015 ASCO
Place of Presentation
Chicago, USA
Year and Date
2015-05-29
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
