| Project/Area Number |
26670503
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Ehime University |
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Principal Investigator |
Ishii Eiichi 愛媛大学, 医学(系)研究科(研究院), 教授 (20176126)
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| Co-Investigator(Kenkyū-buntansha) |
Eguchi Mariko 愛媛大学, 大学院医学系研究科, 准教授 (40420781)
Eguchi Minenori 愛媛大学, 医学部附属病院, 講師 (50420782)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 小児血液学 / 白血病 / 融合遺伝子 |
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| Outline of Final Research Achievements |
Methods for rapid identification of leukemia-specific fusion genes, MLL-AF4 and TEL-AML1, by polymerase chain reaction (PCR) with DNA samples were established. MLL-AF4 and TEL-AML1 are two frequent fusion genes observed in pediatric acute lymphoblastic leukemia and both fusion genes are often generated in utero preceding the development of overt leukemia. In utero generation of these fusion genes could be a possible candidate for preclinical diagnosis of leukemia with these fusion genes. Multiple primers were designed on introns in which most of the translocation breakpoints are present. By setting the distance between two adjacent primers to less than 3kb, expected sizes of PCR products with these primers could be set at less than 6kb length. Establishment of efficient preclinical screening methods of childhood leukemia by early detection of these fusion genes are now pursued as a next step.
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