| Project/Area Number |
26670518
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Manabe Motomu 秋田大学, 大学院医学系研究科, 教授 (30138309)
Horie Yasuo 秋田大学, 大学院医学系研究科, 講師 (30282164)
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| Research Collaborator |
SUZUKI Tomoko
KAGAYA Masami
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 壊疽性膿皮症 / イノシトールリン脂質 / ノックアウトマウス / 炎症性皮膚疾患 / 細胞内小胞輸送系 / PIPKIII / 細胞内小胞輸送 / 空胞形成 / タモキシフェン / PipkIII / オートファージー / 遺伝子改変マウス |
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| Outline of Final Research Achievements |
Pyoderma gangrenosum is a chronic inflammatory skin disease of unknown etiology. To investigate the pathogenic mechanism for pyoderma gangrenosum, we generated mutant mice harboring epidermal specific deletion of type III phosphatidylinositol phosphate kinase (PipkIII). However, the mutant mice died several hours after birth. To overcome this neonatal death and to examine functional roles of PipkIII in the epidermis, we then generated tamoxifen-inducible PipkIII-deficient mice. After tamoxifen application, we found that intensive vacuolization occurred in the epidemis and follicular epithelium. However, we failed to detect dense neutrophilic infiltration in the dermis, which is a characteristic histopathological feature of pyoderma gangrenosum, in the mutant mice. We will examine the role of PipkIII in intracellular transport in the epidermis.
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