Creation of iPS-derived Hepatocyte-based Therapeutic Modality Using Liver Micro-organoids
| Project/Area Number |
26670587
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Osaka University |
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Principal Investigator |
OHASHI KAZUO 大阪大学, 薬学研究科(研究院), 教授 (40364062)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUGUCHI Hiroyuki 大阪大学, 薬学研究科, 教授 (50311387)
YAMADA Masumi 千葉大学, 工学研究科, 准教授 (30546784)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 人工臓器学 / 細胞療法 / 組織工学 / 再生医療 / 肝細胞 / iPS細胞 / 薬物代謝 |
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| Outline of Final Research Achievements |
The aim of this project was to create several fundamental technologies that allow us to create cell-loaded transplantable devices that could be used for cell-based therapies toward liver diseases. The fundamental concept is that cells will be localized after transplantation, instead of scattering the cells after conventional cell infusion-based transplantation procedures.
Hepatocytes are loaded into ultra-thin (<200micrometer in diameter) alginate micro-fibers using micro-fluidic devices and microfluidic technologies for creating liver micro-organoids. We have confirmed liver functions at considerable levels of the liver micro-organoids made of HepG2 cells or iPS-derived hepatocytes with non-parenchymal cells. We have also confirmed that the liver micro-organoids were durable for transplantation procedures on a mouse transplantation experiments.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice.2015
Author(s)
Yusuke Sakai, Kosho Yamanouchi, Kazuo Ohashi, Makiko Koike, Rie Utoh, Hideko Hasegawa, Izumi Muraoka, Takashi Suematsu, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi
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Journal Title
Biomaterials.
Volume: 65
Pages: 66-75
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] CCAAT/enhancer binding protein-mediated regulation of TGFβ receptor 2 expression determines hepatoblast fate decision.2014
Author(s)
Takayama K., Kawabata K., Nagamoto Y., Inamura M., Ohashi K., Okuno H., Yamaguchi T., Tashiro K., Sakurai F., Hayakawa T., Okano T., Furue MK., Mizuguchi H.
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Journal Title
Development,141: 91-100, 2014.
Volume: 141
Pages: 91-100
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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