RNA edition as an escape mechanism of malignant pleural mesothelioma cells from cell stress

• Project/Area Number: 26670624
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Hokkaido University
• Principal Investigator: Kaga Kichizo 北海道大学, 大学病院, 講師 (80224335)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 悪性胸膜中皮腫 / 悪性化 / RNA編集 / 低酸素

Outline of Final Research Achievements

Over expression of COPA, neither edited nor non-edited RNA of inhibited growth of malignant pleural mesothelioma. Over expression did not affect migration and invasion of malignant pleural mesothelioma.
High density culture increased COPA expression. Culture under either hypoxia + low glucose, or hypoxia +high glucose revealed that glucose concentration does not influence COPA expression.

Research Products

• [Journal Article] Comparative genetic analysis of a rare synchronous collision tumor composed of malignant pleural mesothelioma and primary pulmonary adenocarcinoma. (2016)
  • Author(s): Naka T, Hatanaka Y, Marukawa K, Okada H, Hatanaka KC, Sakakibara-Konishi J, Oizumi S, Hida Y, Kaga K, Mitsuhashi T, Matsuno Y
  • Journal Title: Diagn Pathol Volume: 11 Issue: 1 Pages: 1-6
  • DOI: 10.1186/s13000-016-0488-0
  • NAID: 120005766752
  • Peer Reviewed / Open Access
• [Journal Article] Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma (2016)
  • Author(s): Kato T, Lee D, Wu L, Patel P, Young AJ, Wada H, Hu HP, Ujiie H, Kaji M, Kano S, Matsuge S, Domen H, Kaga K, Matsui Y, Kanno H, Hatanaka Y, Hatanaka KC, Matsuno Y, de Perrot M, Yasufuku K
  • Journal Title: Int J Oncol Volume: 49 Issue: 2 Pages: 448-456
  • DOI: 10.3892/ijo.2016.3566
  • NAID: 120006777921
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SORORIN and PLK1 as potential therapeutic targets in malignant pleural mesothelioma (2016)
  • Author(s): Kato T, Lee D, Wu L, Patel P, Young AJ, Wada H, Hu HP, Ujiie H, Kaji M, Kano S, Matsuge S, Domen H, Kanno H, Hatanaka Y, Hatanaka KC, Kaga K, Matsui Y, Matsuno Y, De Perrot M, Yasufuku K
  • Journal Title: Int J Oncol Volume: 49 Issue: 6 Pages: 2411-2420
  • DOI: 10.3892/ijo.2016.3765
  • NAID: 120006777919
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The significance of metastasectomy in patients with metastatic renal cell carcinoma (2015)
  • Author(s): Miyata H, Shinohara N, Murahashi N, Tsuchiya K, Miyajima N, Maruyama S, Abe T, Kaga K, Hirano S, Nonomura K.
  • Journal Title: Hinyokika Kiyo Volume: 61 Pages: 49-54
  • NAID: 120005575176