| Project/Area Number |
26670627
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宗 淳一 岡山大学, 大学病院, 講師 (90559890)
山本 寛斉 岡山大学, 大学病院, 助教 (40467733)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | REIC遺伝子 / 非小細胞肺癌 / 遺伝子治療 / REIC / 肺癌 / 悪性胸膜中皮腫 / NK細胞 / MHC Class I / GRP78 / MHC Class-I / MHC Class1 |
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| Outline of Final Research Achievements |
REIC is down-regulated in many human cancer cells and is considered to function as a tumor suppressor. REIC-expressing adenovirus vector (Ad-REIC) induces endoplasmic reticulum (ER) stress and cancer-specific apoptosis in human cancers. We examined the therapeutic impact of Ad-REIC on non-small cell lung cancer and malignant pleural mesothelioma. We examined the direct anti-tumor effect of Ad-REIC gene therapy on non-small cell lung cancer and malignant pleural mesothelioma cell lines in vitro, and the distant bystander effect using immunocompetent mouse allograft models with bilateral flank tumors. Ad-REIC treatment showed antitumor effect in many lung cancer and malignant pleural mesothelioma cell lines in vitro. In an in vivo model, Ad-REIC treatment inhibited the growth not only of directly treated tumors but also distant untreated tumors. Ad-REIC treatment not only had a direct antitumor effect but also an indirect bystander effect through stimulation of the immune system.
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