New therapeutic strategy for non-small cell lung cancer by REIC gene therapy
Project/Area Number |
26670627
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Respiratory surgery
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Research Institution | Okayama University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
宗 淳一 岡山大学, 大学病院, 講師 (90559890)
山本 寛斉 岡山大学, 大学病院, 助教 (40467733)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | REIC遺伝子 / 非小細胞肺癌 / 遺伝子治療 / REIC / 肺癌 / 悪性胸膜中皮腫 / NK細胞 / MHC Class I / GRP78 / MHC Class-I / MHC Class1 |
Outline of Final Research Achievements |
REIC is down-regulated in many human cancer cells and is considered to function as a tumor suppressor. REIC-expressing adenovirus vector (Ad-REIC) induces endoplasmic reticulum (ER) stress and cancer-specific apoptosis in human cancers. We examined the therapeutic impact of Ad-REIC on non-small cell lung cancer and malignant pleural mesothelioma. We examined the direct anti-tumor effect of Ad-REIC gene therapy on non-small cell lung cancer and malignant pleural mesothelioma cell lines in vitro, and the distant bystander effect using immunocompetent mouse allograft models with bilateral flank tumors. Ad-REIC treatment showed antitumor effect in many lung cancer and malignant pleural mesothelioma cell lines in vitro. In an in vivo model, Ad-REIC treatment inhibited the growth not only of directly treated tumors but also distant untreated tumors. Ad-REIC treatment not only had a direct antitumor effect but also an indirect bystander effect through stimulation of the immune system.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Distant Bystander Effect of REIC/DKK3 Gene Therapy Through Immune System Stimulation in Thoracic Malignancies.2017
Author(s)
Suzawa K, Shien K, Peng H, Sakaguchi M, Watanabe M, Hashida S, Maki Y, Yamamoto H, Tomida S, Soh J, Asano H, Tsukuda K, Nasu Y, Kumon H, Miyoshi S, Toyooka S.
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Journal Title
Anticancer Research
Volume: 37
Pages: 301-307
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Anti-Cancer Effects of REIC/Dkk-3-encoding Adenoviral Vector for the Treatment of Non-small Cell Lung Cancer2014
Author(s)
Shien K, Tanaka N, Watanabe M, Soh J, Sakaguchi M, Matsuo K, Yamamoto H, Furukawa M, Asano H, Tsukuda K, Nasu Y, Huh NH, Miyoshi S, Kumon H, Toyooka S
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Journal Title
PLoS ONE
Volume: 9
Issue: 2
Pages: e87900-e87900
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] The proof of concept of REIC/Dkk-3 gene therapy for thoracic malignancies.2016
Author(s)
Shinichi Toyooka, Ken Suzawa, Kazuhiko Shien, Masakiyo Sakaguchi, Masami Watanabe, Peng Huang, Kei Namba, Hidejiro Torigoe, Hiroki Sato, Junichi Soh, Hiromasa Yamamoto, Yuho Maki, Kazunori Tsukuda, Shuta Tomida, Yasutomo Nasu, Hiromi Kumon, Shinichiro Miyoshi
Organizer
10th AACR-JCA Joint Conference on Breakthroughs in Cancer Research: From Biology to Therapeutics
Place of Presentation
米国
Year and Date
2016-02-16
Related Report
Int'l Joint Research
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[Presentation] Adenovirus-mediated REIC/Dkk-3 gene therapy shows indirect antitumor effects on thoracic malignancies through immune system stimulation2015
Author(s)
Ken Suzawa, Kazuhiko Shien, Masakiyo Sakaguchi, Masami Watanabe, Shinsuke Hashida, Junichi Soh, Hiromasa Yamamoto, Yuho Maki, Kazunori Tsukuda, Yasutomo Nasu, Hiromi Kumon, Shinichiro Miyoshi, Shinichi Toyooka
Organizer
第74回日本癌学会学術総会
Place of Presentation
米国
Year and Date
2015-10-08
Related Report
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[Presentation] Distant bystander effect of REIC/Dkk-3 gene therapy through immune system stimulation in a murine model of thoracic malignancies2015
Author(s)
Ken Suzawa, Kazuhiko Shien, Pen Huang, Masakiyo Sakaguchi, Mototsugu Watanabe, Shinsuke Hashida, Junichi Soh, Hiromasa Yamamoto, Yuho Maki, Hiroaki Asano, Kazunori Tsukuda, Yasutomo Nasu, Hiromi Kumon, Shinichiro Miyoshi, Shinichi Toyooka
Organizer
16th World Conference on Lung Cancer
Place of Presentation
名古屋
Year and Date
2015-09-06
Related Report
Int'l Joint Research