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Phospholipid dynamics in osteoclast fusion

Research Project

Project/Area Number 26670675
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

IRIE Atsushi  公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主任研究員 (10280786)

Co-Investigator(Renkei-kenkyūsha) MURAKAMI Makoto  (公益財団法人)東京都医学総合研究所, 生体分子先端研究分野, プロジェクトリーダー (60276607)
Research Collaborator YAMAMOTO Kei  
MIKI Yoshimi  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords破骨細胞 / リン脂質
Outline of Final Research Achievements

Osteoclasts, responsible for bone resorption, are formed by cell-cell fusion of mononuclear pre-osteoclasts. We found that the cellular content of phospholipids, phosphatidylethanolamine (PE) in particular, was increased during osteoclast differentiation. Furthermore, PE was greatly increased in the cell surface of the osteoclast precursors. Immobilisation of the cell surface PE blocked osteoclast fusion, revealing the importance of PE abundance and distribution. To identify the molecules responsible for these PE dynamics, we screened a wide array of lipid-related genes and found that LPEAT2, ABCB4 and ABCG1 are key players for PE biosynthesis and redistribution. Taken together, our findings demonstrate that the PE dynamics play an essential role in osteoclast fusion.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Phosphatidylethanolamine dynamics are required for osteoclast fusion.2017

    • Author(s)
      Irie, A., Yamamoto, K., Miki, Y., and Murakami, M.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 46715-46715

    • DOI

      10.1038/srep46715

    • NAID

      120006534988

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] リン脂質動態に基づく破骨細胞融合機構の解明2016

    • Author(s)
      入江 敦
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ホスファチジルエタノールアミンは破骨細胞融合に関与する2015

    • Author(s)
      入江 敦
    • Organizer
      第38回日本分子生物学会・第88回日本生化学会
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
  • [Presentation] リン脂質動態に基づく破骨細胞融合機構の解明2015

    • Author(s)
      入江 敦
    • Organizer
      第57回日本脂質生化学会
    • Place of Presentation
      一橋講堂(東京都千代田区)
    • Year and Date
      2015-05-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Phosphatidylethanolamine dynamics in osteoclast fusion.2015

    • Author(s)
      入江 敦
    • Organizer
      6th International Conference on Phospholipase A2 and Lipid Mediators
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2015-02-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 膜脂質動態に基づく破骨細胞融合機構の解明.2015

    • Author(s)
      入江 敦
    • Organizer
      TOBIRA 第4回研究交流フォーラム
    • Place of Presentation
      ソラシティカンファレンスセンター(東京都千代田区)
    • Year and Date
      2015-02-02
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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