Epigenetic influence via sevoflurane exposure to colon tumor cell
| Project/Area Number |
26670685
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Nagoya University |
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Principal Investigator |
ISHIDA Yuki 名古屋大学, 医学部附属病院, 病院助教 (10718315)
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| Co-Investigator(Kenkyū-buntansha) |
SENGA TAKESHI 名古屋大学, 大学院医学系研究科, 准教授 (80419431)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | セボフルラン / トランスクリプトーム / MTT / 吸入麻酔薬 / トランスクリプトソーム網羅解析 |
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| Outline of Final Research Achievements |
Previously we found that sevoflurane inhaled colon tumor cells showed active proliferation compared to none exposure cells. To know the cause of the proliferation, we hypothesized that sevoflurane inhaled HCT116 may change its transcriptomes and epigenetic alterations on DNA. In this study HCT116 were inhaled by 1% sevoflurane for four, eight, twelve hours or none exposure and these mRNAs were read via Next generation sequencing (NGS) Illumina HiSeq. From this sequencing analysis we found that apparently Calcium and Sodium ion channel gene expressions were increased/decreased significantly. Also epigenome related DNA methylation genes were highly expressed in a time dependent manner. We concluded that HCT116 is significantly altered its transcriptome with clinically relevant 1% sevoflurane exposure.
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Report
(3 results)
Research Products
(1 results)
