| Project/Area Number |
26670699
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ZAKOJI Hidenori 山梨大学, 総合研究部, 准教授 (60345717)
SAWADA Norifumi 山梨大学, 総合研究部, 助教 (70402055)
MIYAMOTO Tatsuya 山梨大学, 総合研究部, 助教 (80456459)
IHARA Tatsuya 山梨大学, 医学部附属病院, 診療助教 (90622407)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 下部尿路機能 / 難治性下部尿路機能障害 / 小胞型トランスポーター / 機械センサー / 時計遺伝子 / 難治性 / 下部尿路機能障害 / 過活動膀胱 / 小胞型トランスポータ / ATP / TRPイオンチャネル / Piezo |
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| Outline of Final Research Achievements |
Micturition behabior was examined using a special metabolic cage. Male C57BL/6 mice aged 8-12 weeks old (WT) and male C57BL/6 ClockΔ19/Δ19 mice aged 8 weeks old were used. They were bred under 12-h light/dark conditions for 2 weeks and voiding behavior was investigated by measuring water intake volume, urine volume, urine volume/void, and voiding frequency in metabolic cages in the dark and light periods.ClockΔ19/Δ19 mice showed the phenotype of Nocturia(NC)/Nocternal Polyuria(NP). The ClockΔ19/Δ19 mouse may be used as an animal model of NOC and NP. We investigated the gene expression rhythms of the mechanosensory cation channels(TRPV4, Piezo1), and a main ATP release mediate molecules (ARMM)such as VNUT and Cx26 in the urothelium, in addition to clock genes.Disruption of circadian rhythms in mechano-sensors and ARMM, in addition to abnormalities in clock genes, could be one of the causes of NOC because of the hyper-sensitivity of bladder wall extension.
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