Morphological and experimental analyses of organogenesis and histogenesis of the urinary organs
| Project/Area Number |
26670701
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Shimane University |
|---|
Principal Investigator |
Otani Hiroki 島根大学, 医学部, 教授 (20160533)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
松本 暁洋 島根大学, 医学部, 助教 (70346378)
小川 典子 島根大学, 医学部, 助教 (90598111)
古屋 智英 島根大学, 医学部, 助教 (40457172)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 先天異常 / 泌尿器 / 器官形成 / 組織形成 / 奇形学 |
|---|
| Outline of Final Research Achievements |
Normal and abnormal prenatal development of the urinary organs were analyzed using embryos of genetically modified mice, embryo manipulation, histomorphometric and mathematical methods, with special focus on the organogenesis including ureter elongation and the histogenesis including the total nephron number.
Regarding the organogenesis, it was elucidated that the ureter elongation involves convergent extension mechanism, that interkinetic nuclear migration plays a role in the epithelial cell proliferation, and that Wnt5a/Ror2 signaling plays roles in the development of the ureter and kidney. Regarding the histogenesis, ACTH producing pituitary tumor cell line AtT20 cells were transplanted into mouse embryos by using the exo utero development system, and the organs including the kidney of the resultant adult mice with the introduced tumor cells have been harvested and now are under histological analysis. This experimental system will be useful in the analysis of other organ system.
|
Report
(4 results)
Research Products
(38 results)
-
-
[Journal Article] Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness.2017
Author(s)
Nishita, M., Park, S.-Y., Nishio, T., Kamizaki, K., Wang, Z., Tamada, K., Takumi, T., Hashimoto, R., Otani, H., Pazour, G.J., Hsu, V.W., and Minami, Y.
-
Journal Title
Sci. Rep. 7: 1, 2017.
Volume: 7
Issue: 1
Pages: 1-1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
[Journal Article] Melanocortins Contribute to Sequential Differentiation and Enucleation of Human Erythroblasts via Melanocortin Receptors 1,2 and 52015
Author(s)
Simamura E, Arikawa T, Ikeda T, Shimada H, Shoji H, Masuta H, Nakajima Y, Otani H, Yonekura H, Hatta T
-
Journal Title
PLOS ONE
Volume: 10
Issue: 4
Pages: 1-17
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
[Journal Article] Role of Wnt5a-Ror2 signaling in morphogenesis of the metanephric mesenchyme during ureteric budding2014
Author(s)
Nishita M, Qiao S, Miyamoto M, Okinaka Y, Yamada M, Hashimoto R, Iijima K, Otani H, Hartmann C, Nishinakamura R, Minami Y
-
Journal Title
Mol Cell Biol
Volume: 34
Issue: 16
Pages: 3096-3105
DOI
Related Report
Peer Reviewed / Open Access
-
[Presentation] Interkinetic nuclear migration in the developing esophageal, tracheal, and intestinal epithelia2017
Author(s)
Otani H, Nitta T, Ogawa N, Kaneda R, Saeki Y, Rafiq AM, Jahan E,Regassa DG,, Furuya M, Matsumoto A, Udagawa J, Hatta T
Organizer
第122回日本解剖学会全国学術集会
Place of Presentation
長崎大学坂本キャンパス（長崎市）
Year and Date
2017-03-28
Related Report
-
-
-
-
-
-
-
-
-
-
[Presentation] Interkinetic nuclear migration in the developing endoderm- and mesoderm-origin epithelial tubular structures2016
Author(s)
Otani H, Motoya T, Ogawa N, Nitta T, Rafiq AM, Jahan E, Kaneda R, Furuya M, Matsumoto A, Udagawa J, Hatta T
Organizer
第121回日本解剖学会全国学術集会
Place of Presentation
ビックパレット福島（郡山市）
Year and Date
2016-03-28
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
