| Project/Area Number |
26670765
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Kagoshima University (2015-2016)
Kyushu University (2014) |
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Principal Investigator |
Ieiri Satoshi 鹿児島大学, 医歯学域医学系, 教授 (00363359)
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| Co-Investigator(Kenkyū-buntansha) |
田口 智章 九州大学, 医学研究院, 教授 (20197247)
黒田 達夫 慶應義塾大学, 医学部(信濃町), 教授 (60170130)
下島 直樹 慶應義塾大学, 医学部(信濃町), 講師 (30317151)
炭山 和毅 東京慈恵会医科大学, 医学部, 教授 (90385328)
藤ヶ崎 純子 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究副部長 (60312021)
宮田 潤子 (秋吉潤子 / 宮田 潤子(秋吉潤子)) 九州大学, 大学病院, 助教 (20380412)
三好 きな 九州大学, 医学研究院, 助教 (20621709)
小幡 聡 九州大学, 大学病院, 助教 (30710975)
神保 教広 九州大学, 医学研究院, 共同研究員 (10650559)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 機能性消化管疾患 / ヒルシュスプルング病 / 共焦点型内視鏡 / 神経節細胞 / 筋間神経叢 / ヒルシュスプルング病類縁疾患 / 共焦点内視鏡 / 消化管神経叢 |
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| Outline of Final Research Achievements |
In preceding studies, we identified that some fluorescent agents cloud visualization of the myenteric plexus (MP) with confocal laser endomicroscopy (CLE). In this study, we evaluated the technical feasibility of MP visualization using probe-based CLE (pCLE) with cresyl violet (CV), which has been used clinically for chromoendoscopy. We tested the feasibility of CV-assisted visualization of MP in human surgical specimens of Hirschsprung's disease, wherein the tissue dying and pCLE observation were performed from the serosal side. In the human study, rate of MP visualization by pCLE was evaluated as the primary outcome. We also evaluated the sensitivity and specificity of MP visualization by pCLE, using pathological presence/absence of MP as the gold standard. This study demonstrated the technical feasibility of visualizing the MP in real time by CV-assisted pCLE.
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