| Project/Area Number |
26670794
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸茂 幹雄 兵庫医科大学, 医学部, 講師 (40333950)
小谷 穣治 兵庫医科大学, 医学部, 教授 (80360270)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 敗血症 / 循環不全 / 血管内凝固症候群 / ペプチドマーカー / ショック / 播種性血管内凝固症候群 / ペプチドーム解析 |
|---|
| Outline of Final Research Achievements |
Peptides in the blood of patients suffering from sepsis were inclusively analyzed using the BLOTCHIP―MS method. Twenty patients with sepsis were divided into two groups of patients with and those without a complication of disseminated intravascular coagulation (DIC). By differential analysis of peptides in blood for these groups, we selected thirteen MS peaks as candidate marker peptides for prediction of DIC. By further ROC analysis, eleven sets, each consisting of four peak peptides, were found to show AUC of 1. Among the thirteen peptides, four frequently detected peptides were subjected to precision mass measurement and subsequent MS/MS analysis. Consequently, two peptides of m/z = 2743 and m/z = 2886 were identified to be α2-HS-glycoprotein and fibrinogen β chain, respectively, both of which consist of 27 amino acids. These peptides are expected to be biomarkers for prediction of DIC in patients with sepsis.
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