Biogenesis of sugar moiety of anionic lipopolysaccharide associated with CTD proteins
| Project/Area Number |
26670804
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAYAMA Koji 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80150473)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Masahito 鹿児島大学, 理工学研究科, 准教授 (30333537)
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| Co-Investigator(Renkei-kenkyūsha) |
SHOJI Mikio 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10336175)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯周病 / 細菌 / ＬＰＳ / LPS |
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| Outline of Final Research Achievements |
We constructed a Porphyromonas gingivalis mutant deficient in the WbpD homolog, PGN_0002, which is involved in the Wbp pathway and found that the mutant is deficient in A-LPS, suggesting that P. gingivalis may synthesize UDP-GlcNAc(3NAc)A, for incorporation into A-LPS. Mutants were successfully constructed in 13 genes among the 15 genes encoding putative glycosyltransferases. Biochemical analysis of the mutants suggested that PGN_1240 protein is essential for the synthesis of both O-LPS and A-LPS, PGN_0361 is necessary for A-LPS synthesis, and PGN_0361 and PGN_1239 proteins functionally compensate each other in O-LPS synthesis.
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Report
(3 results)
Research Products
(6 results)
