| Project/Area Number |
26670805
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Fujiwara Naoki 岩手医科大学, 歯学部, 准教授 (20190100)
Otsu Keishi 岩手医科大学, 歯学部, 講師 (60509066)
Kumakami Mika 岩手医科大学, 歯学部, 常任研究員 (30710826)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | エナメル上皮幹細胞 / 切歯 / シグナルセンター / 外エナメル上皮 / 発生 / マウス切歯 / 歯の発生 / 切歯歯胚 |
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| Outline of Final Research Achievements |
In order to examine the mechanisms of apical bud (dental epithelial niche) development in a continuously growing mouse incisor, we had observed incisor germ growth using live cell imaging. We had found a special cell population (dental epithelial bulge of outer enamel epithelium: DEB of OEE), which supply some cells toward incisal end and distal end, in the outer enamel epithelium of labial dental epithelium. First, we attempted to analyze the detail morphology of a developing incisor germ using 3D imaging of sheet laser microscopy and CK14cre/Rosa26-tdTomato. The morphology is similar to a 90 degree rotated molar tooth germ, and exhibit a elongated labial dental epithelium. Next, we observed cell cycle using Fucci mouse and glycogen deposit by immunohistochemistry in DEB of OEE. The results showed that some cells in DEB of OEE showed cessation at G0 and accumulated glycogen. Considering with these results, we presumed that DEB of OEE is an important area for production of apical bud.
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