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Establishment of a novel gene promoter activity detection system for cell isolation and characterization.

Research Project

Project/Area Number 26670815
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionOkayama University

Principal Investigator

KOZAKI Ken-ichi  岡山大学, 大学院医歯薬学総合研究科, 教授 (50270715)

Co-Investigator(Kenkyū-buntansha) SOGAWA Norio  松本歯科大学, 歯学部, 教授 (30236153)
SOGAWA Chiharu  岡山大学, 大学院医歯薬学総合研究科, 講師 (10253022)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords発現制御 / 遺伝子 / 癌 / 発生・分化 / 移植・再生医療
Outline of Final Research Achievements

We established a novel gene promoter activity detection (gPAD) system and utilized it in a cell-based drug screening, in which promoter sequences of marker genes were connected with fluorescent reporter genes and inserted in stable cell clones. Concretely, we established a gPAD system with a promoter sequence of VIM gene that encodes mesenchymal marker vimentin in order to evaluate alteration of epithelial-mesenchymal transition (EMT) program in cancer cells and then performed functional screening using the cell-based reporter system. We established such VIM gPAD system within MKN1 colon cancer cells, performed screening from 328 species of miRNAs, and then identified a novel EMT-inducing microRNA miR-544a. miR-544a was shown to promote EMT through targeting CDH1 gene, encoding an epithelial marker E-cadherin, and targeting AXIN2 gene, encoding a Wnt signaling inhibitory factor, and thus to activate Wnt and TGFβ signaling pathways (Yanaka Y et al., 2015, Carcinogenesis). As above, an example and usefulness of gPAD system in drug screening were shown.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2015 2014 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] miR-544a induces epithelial-mesenchymal transition through the activation of WNT signaling pathway in gastric cancer2015

    • Author(s)
      Yanaka Y, Muramatsu T, Uetake H, Kozaki K, Inazawa J
    • Journal Title

      Carcinogenesis.

      Volume: 36 Issue: 11 Pages: 1363-71

    • DOI

      10.1093/carcin/bgv106

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mitochondrial Apoptosis Pathway and Enhances Chemotherapy-Induced Cytotoxicity.2015

    • Author(s)
      2. Fujiwara N, Inoue J, Kawano T, Tanimoto K, Kozaki K, Inazawa J
    • Journal Title

      Cancer Res.

      Volume: 75 Issue: 18 Pages: 3890-3901

    • DOI

      10.1158/0008-5472.can-15-0257

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 核酸医薬への応用を目指した癌関連microRNAの統合的スクリーニング2015

    • Author(s)
      小崎健一
    • Journal Title

      岡山歯学会雑誌

      Volume: 34 Pages: 1-10

    • Related Report
      2015 Annual Research Report
  • [Journal Article] The expression and clinical significance of connective tissue growth factor in advanced head and neck squamous cell cancer.2014

    • Author(s)
      Kikuchi R, Kikuchi Y, Tsuda H, Maekawa H, Kozaki KI, Imoto I, Tamai S, Shiotani A, Iwaya K, Sakamoto M, Sekiya T, Matsubara O
    • Journal Title

      Human Cell

      Volume: 印刷中 Issue: 3 Pages: 121-128

    • DOI

      10.1007/s13577-014-0092-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] The impact of miRNA-based molecular diagnostics and treatment of NRF2-stabilized tumors.2014

    • Author(s)
      Yamamoto S, Inazawa J, et al.
    • Journal Title

      Mol Cancer Res.

      Volume: 12 Issue: 1 Pages: 58-68

    • DOI

      10.1158/1541-7786.mcr-13-0246-t

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] miR-655とmiR-544aはEMTレポーターシステムにより同定されたEMT制御miRNAである.2015

    • Author(s)
      村松智輝、谷中淑光、原園陽介、小崎健一、稲澤譲治
    • Organizer
      日本癌学会74回総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 高転移腫瘍における統合的解析から同定された新規治療標的経路ハイプシンカスケードの解析2014

    • Author(s)
      村松智輝、小崎健一、井元清哉、山口 類、津田 均、森下真紀、河野辰幸、宮野 悟、稲澤譲治
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 (横浜市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞ベースのリポーターアッセイを用いた胃癌でvimentinを発現上昇させるmiRNAの探索2014

    • Author(s)
      谷中淑光、小崎健一、稲澤譲治
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 (横浜市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] NNC05-2090の抗アロディニア効果とその他の薬理作用について2014

    • Author(s)
      十川千春、秦泉寺紋子、大山和美、宮脇卓也、森田克也、十川紀夫、小崎健一
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場 (福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 新規癌抑制性microRNAとしてのMir-634の同定2014

    • Author(s)
      藤原直人、井上 純、河野辰幸、小崎健一、稲澤譲治
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 (横浜市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] マイクロRNAを基盤としたNRF2活性化癌に対する診断・治療法の確立2014

    • Author(s)
      井上 純、山本信祐、藤原直人、河野辰幸、小村 健、小崎健一、稲澤譲治
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 (横浜市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] MicroRNA-634 induces caspase-dependent apoptosis by targeting anti-apopotic and mitochondria-related genes in human cancer cells.2014

    • Author(s)
      Fujiwara N., Inoue J., Kawano T., Kozaki K. and Inazawa J.
    • Organizer
      American Association for Cancer Research, 105th Annual Meeting 2014
    • Place of Presentation
      San Diego Convention Center (San Diego, CA, USA)
    • Year and Date
      2014-04-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Exploring target gene(s) within chromosome 19-amplification detected in a subclone from metastatic tumors in mouse transplantable OSCC.2014

    • Author(s)
      Muramatsu T., Kozaki K., Imoto S., Yamaguchi R., Tsuda H., Kawano T., Miyano S. and Inazawa J.
    • Organizer
      American Association for Cancer Research, 105th Annual Meeting 2014
    • Place of Presentation
      San Diego Convention Center (San Diego, CA, USA)
    • Year and Date
      2014-04-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Genome-wide screening of DNA methylation markers to predict the presence of lymph node metastasis in esophageal squamous cell carcinoma.2014

    • Author(s)
      Kozaki K., Nagata H., Tanimoto K., Fujiwara N., Imoto S., Ichikawa D., Miyano S., Kawano T., Otsuji E. and Inazawa J.
    • Organizer
      American Association for Cancer Research, 105th Annual Meeting 2014
    • Place of Presentation
      San Diego Convention Center (San Diego, CA, USA)
    • Year and Date
      2014-04-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 食道扁平上皮癌におけるリンパ節転移関連DNAメチル化マーカーの網羅的探索2014

    • Author(s)
      永田啓明、小松周平、市川大輔、小西博貴、塩崎 敦、藤原 斉、森村 玲、村山康利、栗生宣明、生駒久視、中西正芳、岡本和真、阪倉長平、河野辰幸、宮野 悟、小崎健一、稲澤譲治、大辻英吾
    • Organizer
      第114回日本外科学会定期学術集会
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2014-04-04
    • Related Report
      2014 Research-status Report
  • [Remarks] 岡山大学 研究者カタログ

    • URL

      http://soran.cc.okayama-u.ac.jp/view?l=catalog&u=567f928b353af3e274506e4da22f6611

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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