Establishment of a novel gene promoter activity detection system for cell isolation and characterization.
| Project/Area Number |
26670815
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Okayama University |
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Principal Investigator |
KOZAKI Ken-ichi 岡山大学, 大学院医歯薬学総合研究科, 教授 (50270715)
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| Co-Investigator(Kenkyū-buntansha) |
SOGAWA Norio 松本歯科大学, 歯学部, 教授 (30236153)
SOGAWA Chiharu 岡山大学, 大学院医歯薬学総合研究科, 講師 (10253022)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 発現制御 / 遺伝子 / 癌 / 発生・分化 / 移植・再生医療 |
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| Outline of Final Research Achievements |
We established a novel gene promoter activity detection (gPAD) system and utilized it in a cell-based drug screening, in which promoter sequences of marker genes were connected with fluorescent reporter genes and inserted in stable cell clones. Concretely, we established a gPAD system with a promoter sequence of VIM gene that encodes mesenchymal marker vimentin in order to evaluate alteration of epithelial-mesenchymal transition (EMT) program in cancer cells and then performed functional screening using the cell-based reporter system. We established such VIM gPAD system within MKN1 colon cancer cells, performed screening from 328 species of miRNAs, and then identified a novel EMT-inducing microRNA miR-544a. miR-544a was shown to promote EMT through targeting CDH1 gene, encoding an epithelial marker E-cadherin, and targeting AXIN2 gene, encoding a Wnt signaling inhibitory factor, and thus to activate Wnt and TGFβ signaling pathways (Yanaka Y et al., 2015, Carcinogenesis). As above, an example and usefulness of gPAD system in drug screening were shown.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] The expression and clinical significance of connective tissue growth factor in advanced head and neck squamous cell cancer.2014
Author(s)
Kikuchi R, Kikuchi Y, Tsuda H, Maekawa H, Kozaki KI, Imoto I, Tamai S, Shiotani A, Iwaya K, Sakamoto M, Sekiya T, Matsubara O
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Journal Title
Human Cell
Volume: 印刷中
Issue: 3
Pages: 121-128
DOI
Related Report
Peer Reviewed
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[Presentation] Genome-wide screening of DNA methylation markers to predict the presence of lymph node metastasis in esophageal squamous cell carcinoma.2014
Author(s)
Kozaki K., Nagata H., Tanimoto K., Fujiwara N., Imoto S., Ichikawa D., Miyano S., Kawano T., Otsuji E. and Inazawa J.
Organizer
American Association for Cancer Research, 105th Annual Meeting 2014
Place of Presentation
San Diego Convention Center (San Diego, CA, USA)
Year and Date
2014-04-07
Related Report
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