Investigation for the receptor of dental pulp cell-derived tumor necrosis factor-alpha inducing factor (DPTIF)

• Project/Area Number: 26670824
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: NISHIMURA FUSANORI 九州大学, 歯学研究院, 教授 (80208222)
• Co-Investigator(Kenkyū-buntansha): 讃井 彰一 九州大学, 大学病院, 講師 (70507780) ; 福田 隆男 九州大学, 歯学研究院, 助教 (80507781)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯髄細胞 / 急性炎症 / マクロファージ / TNF-α / 内毒素 / 歯髄炎症 / 急性歯髄炎 / TNF-alpha

Outline of Final Research Achievements

We previously found that dental pulp cells produces molecules inducing tumor necrosis factor alpha from macrophages, and named this factor Dental Pulp Cell-derived Tumor Necrosis Factor-α Inducing Factor: DPTIF. In this study, we aimed to unvail the nature of this molecule and to investigate the posibble receptor and/or binding proteins. The results indicated that DPTIF contains trypsin sensitive protenous molecule but is associated with extracelluler microvesicles. Therefore, it appeared that DPTIF is directly incorporated into macrophages rather than binding to the cell surface receptor.

Research Products

• [Journal Article] Angiopoietin-like protein 2 is a positive regulator of osteoblast differentiation. (2017)
  • Author(s): Takano A, Fukuda T, Shinjo T, Iwashita M, Matsuzaki E, Yamamichi K, Takeshita M, Sanui T, Nishimura F.
  • Journal Title: Metabolism Volume: 69 Pages: 157-170
  • DOI: 10.1016/j.metabol.2017.01.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells (2016)
  • Author(s): Hashimoto, Y,. Kobayashi, M., Matsuzaki, E., Higashi, K., Takahashi-Yanaga, F., Takano, A., Hirata, M. and Nishimura, F.
  • Journal Title: Cell Biol. Int. Volume: 40 Issue: 10 Pages: 1129-1136
  • DOI: 10.1002/cbin.10652
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sphingosine-1-phosphate/S1PR2-mediated signaling triggers Smad1/5/8 phosphorylation and thereby induces Runx2 expression in osteoblasts (2016)
  • Author(s): Higashi, K., Matsuzaki, E., Hashimoto, Y., Takahashi-Yanaga, F., Takano, A., Anan, H., Hirata, M. and Nishimura, F.
  • Journal Title: Bone Volume: 93 Pages: 1-11
  • DOI: 10.1016/j.bone.2016.09.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant