| Project/Area Number |
26670850
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
Endo Kazuhiko 北海道医療大学, 歯学部, 教授 (70168821)
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| Co-Investigator(Kenkyū-buntansha) |
建部 二三 北海道医療大学, 歯学部, 助教 (10534448)
門 貴司 北海道医療大学, 歯学部, 講師 (20632540)
古市 保志 北海道医療大学, 歯学部, 教授 (80305143)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | バイオセンサー / 抗原-抗体反応 / 歯周病原菌 / 歯周病源菌 / QCM / チタン電極 / 化学修飾 / チタン |
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| Outline of Final Research Achievements |
The objective of this study was to develop a novel biosensor which detects pathogenic bacteria as well as pathogenic factors released from the bacteria for providing a diagnostic method of periodontal diseases.
The titanium sensor on the quartz crystal with K6F peptide antibody was made and measured the concentration of K6F peptide which was one of the degradation products of human gingival fibroblasts by a pathogenic factor (Kgp) released from P. gingivalis. A straight line relation was obtained between the resonant frequency change of the quartz crystal immobilized with K6F peptide antibody and the logarithm of the concentration of K6F peptide in the range from 10 ng/ml to 1,000 ng/ml. The detection limit of K6F peptide for the potential measurement method using the titanium wire sensor immobilized with K6F peptide antibody was approximately 5 mg/ml, which was significantly higher than that for quartz crystal microbalance method.
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