| Project/Area Number |
26670851
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Fujiwara Naoki 岩手医科大学, 歯学部, 准教授 (20190100)
OTSU Keishi 岩手医科大学, 歯学部, 講師 (60509066)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 休眠ホルモン / 細胞の保存 / 組織の保存 / ATP / 細胞周期 |
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| Outline of Final Research Achievements |
The aim of this study is the development of novel cell-tissue preservation solution using diapause hormone, Yamamarin,secreted from dormant insects. We evaluated the effect of Yamamarin on the intracellular ATP concentration, cell cycle and cell proliferation in dental enamel epithelial cells. Administration of Yamamarin induced the decrease of intracellular ATP concentration and the arrest of cell cycle and proliferation. However, in some cells apoptosis was induced and large variation was seen in the results. Based on these results, we speculated that the large variation was caused by the differences of sensitivity for cell toxicity of Yamamarin in each cell. It is necessary to reduce the cell toxicity and to render Yamamarin water-soluble in the future.
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