• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pediatric dentistry and Chronomedicine

Research Project

Project/Area Number 26670878
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionHokkaido University

Principal Investigator

Nishide Shinya  北海道大学, 医学(系)研究科(研究院), 助教 (40451398)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords炎症反応 / 概日リズム / 蛍光イメージング / 歯髄炎
Outline of Final Research Achievements

Circadian rhythms are crucial factors in the regulation of a wide range of physiological processes. The systemic circadian system can be broken down into cellular rhythms, which are maintained by periodic changes in the status of a set of clock genes and proteins. Such proteins oscillate not only at the levels of their expression but also at those of posttranslational modification.
In this study, I have constructed fluorescent biosensors for clock proteins, CLOCK, and BMAL1, and observed their subcellular localization and interaction in living cells. CLOCK localized in the cytosol as well as the nucleus, and cytosolic CLOCK accumulated in the endoplasmic reticulum (ER). Deletion of N- or C-terminus of CLOCK changed its subcellular distribution and colocalization with the ER marker in a deleted region-dependent manner.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015

All Presentation (7 results) (of which Invited: 1 results)

  • [Presentation] ライブセルイメージングの口腔疾患予防への応用2016

    • Author(s)
      西出真也
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 蛍光バイオセンサーを用いた時計タンパク質BMAL1-CLOCKの細胞内動態の解析2016

    • Author(s)
      1.西出真也、藤岡容一朗、堀内浩水、堀口美香、佐藤絢、ネパール プラバ、王セイ、南保明日香、大場雄介
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2016-06-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Subcellular localization of the clock proteins, BMAL1 and CLOCK2016

    • Author(s)
      Nishide S, Fujioka Y, Nanbo A, Ohba Y
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Circadian profiling of a dimerization of BMAL1 and CLOCK and their subcellular localization by FRET biosensor2015

    • Author(s)
      Nishide S
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 蛍光イメージングを用いた時計タンパク質の細胞内局在解析2015

    • Author(s)
      西出真也、藤岡容一朗、南保明日香、大場雄介
    • Organizer
      第95回北海道医学大会生理系分科会
    • Place of Presentation
      旭川医科大学(北海道旭川市)
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 唾液中ミュータンス菌の日内変動2015

    • Author(s)
      西出真也、本郷博久、吉原俊博、八若保孝
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Circadian profiling of an interaction between BMAL1 and CLOCK by FRET bioimaging2015

    • Author(s)
      Shin-ya Nishide, Yoichiro Fujioka, Asuka Nanbo, Yusuke Ohba
    • Organizer
      第92回 日本生理学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi