| Project/Area Number |
26670878
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Nishide Shinya 北海道大学, 医学(系)研究科(研究院), 助教 (40451398)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 炎症反応 / 概日リズム / 蛍光イメージング / 歯髄炎 |
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| Outline of Final Research Achievements |
Circadian rhythms are crucial factors in the regulation of a wide range of physiological processes. The systemic circadian system can be broken down into cellular rhythms, which are maintained by periodic changes in the status of a set of clock genes and proteins. Such proteins oscillate not only at the levels of their expression but also at those of posttranslational modification.
In this study, I have constructed fluorescent biosensors for clock proteins, CLOCK, and BMAL1, and observed their subcellular localization and interaction in living cells. CLOCK localized in the cytosol as well as the nucleus, and cytosolic CLOCK accumulated in the endoplasmic reticulum (ER). Deletion of N- or C-terminus of CLOCK changed its subcellular distribution and colocalization with the ER marker in a deleted region-dependent manner.
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