| Project/Area Number |
26670908
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAHARA Taka 日本歯科大学, 生命歯学部, 教授 (10366768)
OHYAMA Akihiro 日本歯科大学, 生命歯学部, 准教授 (90538232)
TOYOMURA Junko 日本歯科大学, 生命歯学部, 助教 (80645630)
KAWAKAMI Miyuki 日本歯科大学, 新潟生命歯学部, 助教 (40707981)
TOMINAGA Noriko 日本歯科大学, 生命歯学部, 助教 (90546532)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ヒト唾液腺 / 歯肉上皮幹細胞 / 共培養 / 3次元的唾液腺 / 唾液腺介在部 / HIF-1alpha / VEGF / 移植 / ヒト歯肉上皮幹細胞 / 唾液腺線維芽細胞 / 3次元唾液腺構築 / HIF-1α / 介在部 / 唾液腺再生 / 誘導分化 / 上皮間葉共培養 / コラーゲンスポンジ |
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| Outline of Final Research Achievements |
First, induction decided to make a salivary gland differentiate from a stem cell of the BFP which has no infection. I succeeded in this, then an isolation an epithelial stem cell from a fragment of a gingival tissue by the Friedenstein method. In order to induced differentiation salivary gland epithelial cell, co-cultivation performed a human salivary gland origin fibroblast and isolated epithelial stem cell of gingival tissue. I succeeded to get salivary epithelial cell, then we try to build a three-dimensional salivary gland by a circumfusion culture. We isolated intercalated duct from these reconstructed salivary gland under a microscope. A three-dimensional salivary gland can be built voluntarily from this interstitial segment. It was revealed that the intercalated duct produces a great deal of HIF-1 α and VEGF. Now, fragment of intercalated duct was implanted under the oral mucous membrane of scid mouse.
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