| Budget Amount *help |
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Outline of Final Research Achievements |
In this project, we focused on cyanobacterial photoreceptors, cyanobacteriochromes (CBCRs), to develop small photo-switches and fluorescent probes that bind biliverdin (BV) and absorb far-red light. Because BV is an intrinsic chromophore in mammalian cells and far-red light can penetrate into deep animal tissues, BV-binding ones are beneficial for optogenetics and bio-imaging tools. We succeeded in discovering novel BV-binding CBCRs showing far-red/orange reversible photoconversion and in developing BV-binding CBCRs based on non-BV-binding ones to introduce several mutations. Further, we also identified dark-reversion type CBCRs and a CBCR with large spectral shift. Based on these molecules, we succeeded in light regulation of cAMP synthesis in vitro and far-red fluorescence detection from mammalian cells.
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