Development of far-red absorbing fluorescent probes and photoconvertible switches for organ-level bio-imaging and optogenetics
| Project/Area Number |
26702036
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Shizuoka University |
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Principal Investigator |
Narikawa Rei 静岡大学, 理学部, 講師 (30456181)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Keywords | オプトジェネティクス / 遠赤色光 / cAMP / シアノバクテリオクロム / 蛍光バイオイメージング / 蛍光プローブ |
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| Outline of Final Research Achievements |
In this project, we focused on cyanobacterial photoreceptors, cyanobacteriochromes (CBCRs), to develop small photo-switches and fluorescent probes that bind biliverdin (BV) and absorb far-red light. Because BV is an intrinsic chromophore in mammalian cells and far-red light can penetrate into deep animal tissues, BV-binding ones are beneficial for optogenetics and bio-imaging tools. We succeeded in discovering novel BV-binding CBCRs showing far-red/orange reversible photoconversion and in developing BV-binding CBCRs based on non-BV-binding ones to introduce several mutations. Further, we also identified dark-reversion type CBCRs and a CBCR with large spectral shift. Based on these molecules, we succeeded in light regulation of cAMP synthesis in vitro and far-red fluorescence detection from mammalian cells.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] シアノバクテリアの光応答戦略2016
Author(s)
成川礼
Organizer
2016年度日本ブランクトン学会春期シンポジウム
Place of Presentation
東京大学本郷キャンパス（東京都文京区）
Year and Date
2016-03-14
Related Report
Invited
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