| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
|
|---|
| Outline of Final Research Achievements |
Fluorescein-modified compounds were prepared and added to the transfected cells, and a marked fluorescence enhancement of fluorescein was observed in cells expressing PLE.
We generated a transgenic fly expressing PLE only in specific cells in the expression-dependent manner of GAL4, and when the above compound was administered to the isolated brain, tissue-specific fluorescence was observed.
In order to examine whether behavior control at an individual level is possible, Drosophila expressing PLE specifically for dopamine cells was created. A compound to which a modifying group was added to a dopamine synthase inhibitor was administered by oral administration, but no change in sleep awakening amount was observed.
|
