Elucidation of rupture mechanisms of aortas by observation of their microstructure at rupture site

• Project/Area Number: 26709002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Materials/Mechanics of materials
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Sugita Shukei 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (20532104)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000); Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
• Keywords: バイオメカニクス / 細胞・組織 / 血管壁 / 破壊メカニズム / コラーゲン / エラスチン / 破壊現象

Outline of Final Research Achievements

The mortality rate due to the rupture of the aortic aneurysm is still high. It is essential to investigate the rupture mechanism. In this study, simultaneous observation of the rupture phenomenon and microstructure of the aorta was performed to provide valuable insights into the rupture mechanisms. Porcine thoracic aortas were biaxially stretched under a microscope. The results showed that local density of collagen fibers was lower at the crack initiation sites. Local density of elastin and connection between elastin and collagen fibers were not related to the rupture sites. Basic researches to establish methods for quantification of sub-type of collagen fibers and observation of rupture of cylindrical shape of the aorta under a microscope were also performed.

Research Products

• [Journal Article] Local distribution of collagen fibers determines crack initiation site and its propagation direction during aortic rupture (2018)
  • Author(s): Sugita Shukei、Matsumoto Takeo
  • Journal Title: Biomechanics and Modeling in Mechanobiology Volume: 17 Issue: 2 Pages: 577-587
  • DOI: 10.1007/s10237-017-0979-2
  • Peer Reviewed
• [Journal Article] Multiphoton microscopy observations of 3D elastin and collagen fiber microstructure changes during pressurization in aortic media (2016)
  • Author(s): Sugita Shukei、Matsumoto Takeo
  • Journal Title: Biomechanics and Modeling in Mechanobiology Volume: 16 Issue: 3 Pages: 763-773
  • DOI: 10.1007/s10237-016-0851-9
  • Peer Reviewed
• [Presentation] Microscopic deformation of the aorta during pressurization based on SHG and two-photon microscopy (2018)
  • Author(s): S. Sugita, Masaya Katoh, Masanori Nakamura, and T. Matsumoto
  • Organizer: The 8th World Congress of Biomechanics
  • Int'l Joint Research
• [Presentation] コラーゲン線維の蛇行度を考慮した血管壁の構成法則 －大動脈瘤破裂予測に向けて－ (2018)
  • Author(s): 今岡健汰朗，中村匡徳，杉山由恵，杉田修啓
  • Organizer: ライフサポート学会第27回フロンティア講演会
• [Presentation] Observation of elastin and collagen fibers in the thoracic aorta under multiphoton microscope during pressurization (2017)
  • Author(s): S. Sugita, T. Matsumoto
  • Organizer: BIT7s 5th Annual Congress of Analytix-2017
  • Place of Presentation: Fukuoka
  • Year and Date: 2017-03-22
  • Int'l Joint Research / Invited
• [Presentation] Relation between Direction of Elastin Fibers and Principal Stress in the Aortic Media (2017)
  • Author(s): Asaka Yamada, Shukei Sugita, Takeo Matsumoto
  • Organizer: The 1st ABiS Symposium
  • Place of Presentation: 東岡崎
  • Year and Date: 2017-02-19
• [Presentation] 大動脈中膜内エラスチン・コラーゲン線維の加圧時の微細構造変化－弾性板層と平滑筋細胞層による差異 (2017)
  • Author(s): 杉田修啓，松本健郎
  • Organizer: 日本機械学会 第29回バイオエンジニアリング講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2017-01-19
• [Presentation] Deformation of elastin and collagen fibers in the aortic media due to changes in pressure (2017)
  • Author(s): S. Sugita, T. Matsumoto
  • Organizer: XXVI Congress of the International Society of Biomechanics
  • Int'l Joint Research
• [Presentation] 第二次高調波発生光によるコラーゲン線維のType I-Type III割合の定量化 (2017)
  • Author(s): 鈴村拓也，杉田修啓
  • Organizer: 日本機械学会 第28回バイオフロンティア講演会
• [Presentation] 血管壁破壊に対するエラスチン線維とコラーゲン線維の結合の影響 (2017)
  • Author(s): 岡田成右，中村匡徳，松本健郎，杉田修啓
  • Organizer: 日本機械学会 第28回バイオフロンティア講演会
• [Presentation] 血管の二光子顕微鏡下全周観察装置の開発と内圧負荷時のコラーゲン線維観察への適用 (2017)
  • Author(s): 加藤雅也，杉田修啓
  • Organizer: 日本機械学会 第30回バイオエンジニアリング講演会
• [Presentation] 胸大動脈内の細胞外マトリックスの引張負荷変形に関する研究 (2017)
  • Author(s): 上間大輝，杉田修啓
  • Organizer: 日本機械学会東海支部第47回学生員卒業研究発表講演会
  • Place of Presentation: 浜松
• [Presentation] コラーゲン線維のType I-Type III割合の定量化に関する基礎研究 (2017)
  • Author(s): 鈴村拓也，杉田修啓
  • Organizer: 日本機械学会東海支部第47回学生員卒業研究発表講演会
  • Place of Presentation: 浜松
• [Presentation] 大動脈中膜内の応力状態とエラスチン線維走行方向との関連 (2016)
  • Author(s): 山田麻加，杉田修啓，松本健郎
  • Organizer: 日本機械学会 第27回バイオフロンティア講演会
  • Place of Presentation: 札幌
  • Year and Date: 2016-10-22
• [Presentation] 引張負荷時の大動脈中膜内エラスチン・コラーゲン線維の多光子顕微鏡による観察 (2016)
  • Author(s): 岡田成右，杉田修啓，松本健郎
  • Organizer: 日本機械学会東海支部第46回学生員卒業研究発表講演会
  • Place of Presentation: 八草
  • Year and Date: 2016-03-16
• [Presentation] 多光子顕微鏡によるエラスチン線維の血管中膜内走行方向の基礎的解析 (2016)
  • Author(s): 山田麻加，杉田修啓，松本健郎
  • Organizer: 日本機械学会東海支部第46回学生員卒業研究発表講演会
  • Place of Presentation: 八草
  • Year and Date: 2016-03-16
• [Presentation] Microscopic deformation of porcine thoracic aortas until failure during biaxial stretch as a model of aortic rupture (2015)
  • Author(s): S. Sugita, T. Matsumoto
  • Organizer: The International Conference on Advanced Technology in Experimental Mechanics 2015
  • Place of Presentation: Toyohashi
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Difference in mechanical properties of collagen fibers in the media and the adventitia of the porcine thoracic aorta (2015)
  • Author(s): S. Sugita, H. Narita, T. Matsumoto
  • Organizer: The 8th Asia Pacific Conference on Biomechanics
  • Place of Presentation: Sapporo
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] Morphological characterization of collagen fibers at the crack initiation sites in biaxially stretched porcine thoracic aortas toward clarification of aneurysm rupture mechanism (2015)
  • Author(s): S. Sugita, T. Matsumoto
  • Organizer: Summer Biomechanics, Bioengineering, and Biotransport Conference
  • Place of Presentation: Salt Lake City
  • Year and Date: 2015-06-17
  • Int'l Joint Research
• [Presentation] 大動脈中膜および外膜由来コラーゲン線維の引張試験に伴う内部微細線維の配向変化 (2015)
  • Author(s): 成田 大興，杉田修啓，松本健郎
  • Organizer: 第24回ライフサポート学会 フロンティア講演会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-05 – 2015-03-06
• [Presentation] コラーゲン線維が2軸引張下の薄切大動脈試料の亀裂開始位置と亀裂伝播路に与える影響 (2015)
  • Author(s): 杉田修啓，松本健郎
  • Organizer: 日本機械学会 第27回バイオエンジニアリング講演会
  • Place of Presentation: 新潟
  • Year and Date: 2015-01-09 – 2015-01-10
• [Remarks] 名古屋工業大学 医用生体工学研究室 研究紹介
  • URL: http://biomech.web.nitech.ac.jp/research/research.html
• [Remarks] 名古屋工業大学バイオメカニクス研究室 研究紹介
  • URL: http://biomech.web.nitech.ac.jp/Research.html