Possible mechanism of virus inactivation by disinfections based on proteome analysis

• Project/Area Number: 26709037
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Kyoto University
• Principal Investigator: Yasuhiro Asada 京都大学, 工学(系)研究科(研究院), 助教 (60610524)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
• Keywords: 上水道 / 健康関連微生物 / 消毒処理 / ウイルス不活化 / プロテオーム解析 / 病原ウイルス / 化学酸化処理 / 感染性ウイルス / タンパク質

Outline of Final Research Achievements

The objectives of this study are to compare and to evaluate inactivation mechanisms by disinfection processes (Chlorination, Ozonation, UV radiation, Advanced Oxidation Process) based on gene analysis and proteome analysis. First, qPCR,PMA-qPCR, ICC-qPCR techniques were employed to determine the inactivation effect. Hydroxyl radicals affected on virus inactivation. And Ozonation is more effective on virus inactivation than other disinfection processes. Also, the obtained results of proteome analysis indicated that damages of proteins are different among Hydroxyl radicals , Chlorination, UV radiation, and ozonation.

Research Products

• [Presentation] プロテオーム解析を用いた酸化処理によるウイルスタンパク質損傷の評価 (2017)
  • Author(s): 藏岡凌平、浅田安廣、榊原崇、越後信哉、伊藤禎彦
  • Organizer: 日本オゾン協会 第26回年次研究講演会
  • Place of Presentation: 東京都水道局 研修・開発センター，東京都
  • Year and Date: 2017-06-15
• [Presentation] Comparison of Two-Dimensional Gel Electrophoresis -based and Mass Spectrometry-based Proteome Analysis of Virus Protein (2017)
  • Author(s): Kuraoka R, Asada Y, Sakakibara T, Echigo S, Itoh S
  • Organizer: The 19th International Symposium on Health -Related Water Microbiology
  • Place of Presentation: Chapel Hill, America
  • Year and Date: 2017-05-15
  • Int'l Joint Research
• [Presentation] ウイルス不活化に対してヒドロキシルラジカルが及ぼす影響 (2017)
  • Author(s): 榊原崇、浅田安廣、越後信哉、蔵岡凌平、伊藤禎彦
  • Organizer: 第51回日本水環境学会年会
  • Place of Presentation: 熊本大学，熊本市
  • Year and Date: 2017-03-15
• [Presentation] Possible mechanism of adenovirus inactivation examined by quantitative PCR and proteomics approach (2016)
  • Author(s): Yamaguchi T, Asada Y, Echigo S, Itoh S
  • Organizer: the 5th Food and Environmental Virology conference
  • Place of Presentation: Kusatsu, Japan
  • Year and Date: 2016-09-13
  • Int'l Joint Research
• [Presentation] ウイルス不活化メカニズム推定に向けたプロテオーム解析手法の適用 (2016)
  • Author(s): 山口達也
  • Organizer: 第50回日本水環境学会年会
  • Place of Presentation: アスティとくしま(徳島県徳島市)
  • Year and Date: 2016-03-16