| Budget Amount *help |
¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2014: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we developed cell assay technology for muscle atrophy. We developed a skeletal muscle tissue chip with a 3D muscle tissue on a microdevice and developed a muscle atrophy model by inducing atrophy to it. Specifically, we succeeded in constructing a three-dimensional tissue that contracts in response to electrical stimulation using mouse and human skeletal muscle cells. We found that the muscle atrophy-related genes were highly expressed and their contractile power significantly decreased by adding the compound to the culture medium of those tissues. Furthermore, we showed that the developed muscle atrophy model was capable of searching for substances that suppress the decrease in contractile force.
|
