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Development of 3D Imaging Cell Sorting Method

Research Project

Project/Area Number 26709064
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Shinji Sugiura  国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (10399496)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords細胞分離 / ハイドロゲル / 癌 / 細胞培養 / 包埋培養 / 画像解析 / 生体材料 / 細胞 / バイオ関連機器
Outline of Final Research Achievements

In a conventional cell separation method such as a flow cytometer, cells are separated using a surface antigen as a marker. On the other hand, the markers for many heterogeneous cell populations such as cancer tissues and stem cell culture systems have been undefined yet, and it is expected to develop a method for separating cells by separately analyzing cell functions for each cell. In this study, we developed the "3D imaging cell sorting method" which separates cells using the photodegradable hydrogel using cell morphology as an indicator for cellular function under three dimensional culture.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (20 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 3 results)

  • [Journal Article] がんの多様性2016

    • Author(s)
      田村 磨聖、杉浦 慎治
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 82-82

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment2015

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
    • Journal Title

      Scientific reports

      Volume: 5 Issue: 1 Pages: 15060-15060

    • DOI

      10.1038/srep15060

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 光分解性ゲルを用いたがん細胞選抜システムの開発2017

    • Author(s)
      杉浦慎治
    • Organizer
      CBI学会2017年大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ハイドロゲル包埋培養系から生きたまま細胞を選抜する自動細胞分離装置の開発2017

    • Author(s)
      杉浦慎治、田村磨聖、山平真也、佐藤 琢、高木 俊之、須丸 公雄、金森 敏幸、渋田真結、 蟹江 慧、加藤竜司、柳沢真澄、松井裕史
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Optical cell manipulation using biomaterial-based photodegradable hydrogels2016

    • Author(s)
      Shinji Sugiura
    • Organizer
      Fall Symposium 2016 of KIChE
    • Place of Presentation
      Korea University, Daejon, Korea
    • Year and Date
      2016-10-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of an optical cell separation based on a cellular shape using click-crosslinkable and photodegradable gelatin hydrogels2016

    • Author(s)
      Masato Tamura, Shinji Sugiura, Taku Satoh, Toshiyuki Takagi, Toshiyuki Kanamori, Mayu Shibuta, Kei Kanie, Ryuji Kato, Hirofumi Matsui, Masumi Yanagisawa
    • Organizer
      The Eighth International Conference on Microtechnologies in Medicine and Biology (MMB 2016)
    • Place of Presentation
      Seoul National University、Seoul、韓国
    • Year and Date
      2016-04-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 『高スループット細胞分離技術を用いた「がん細胞薬剤感受性検査装置」の事業化』2016

    • Author(s)
      杉浦慎治
    • Organizer
      つくばプロモーションイベント「健康長寿 Biz-seeds」
    • Place of Presentation
      常陽銀行、つくば
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光分解性ゲルを用いた細胞操作技術の開発2016

    • Author(s)
      杉浦 慎治
    • Organizer
      Bio Japan2016
    • Place of Presentation
      パシフィコ横浜、横浜
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Development of an optical cell separation based on a cellular shape using click-crosslinkable and photodegradable gelatin hydrogels2016

    • Author(s)
      Masato Tamura, Shinji Sugiura, Taku Satoh, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori, Mayu Shibuta, Kei Kanie, Ryuji Kato, Hirofumi Matsui, Masumi Yanagisawa
    • Organizer
      MMB 2016
    • Place of Presentation
      Seoul University, Seoul, Korea
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Click-crosslinkable and photodegradable gelatin hydrogels for cell separation2016

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori,
    • Organizer
      ICBS2016
    • Place of Presentation
      東京大学、東京
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Micropatterned Three-Dimensional Culture in Click-Crosslinkable and Photodegradable Gelatin Hydrogels2015

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru & Toshiyuki Kanamori
    • Organizer
      26th 2015 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya University、Nagoya
    • Year and Date
      2015-11-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optical cell seraration from 3D culture environment in photodegradable hydrogels for pure culture techniques2014

    • Author(s)
      M. Tamura, F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, H. Matsui and T. Kanamori
    • Organizer
      EMBS Micro and Nanotechnology in Medicine Conference
    • Place of Presentation
      Oahu
    • Year and Date
      2014-12-08 – 2014-12-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Photodegradable hydrogels for optical cell separation from three - dimensional environment2014

    • Author(s)
      M. Tamura, F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, H. Matsui and T. Kanamori
    • Organizer
      The 10th SPSJ International Polymer Conference (IPC 2014)
    • Place of Presentation
      Tsukuba
    • Year and Date
      2014-12-02 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optical cell separation with photodegradable hydrogels from three- dimensional environment for pure culture techniques2014

    • Author(s)
      M. Tamura, F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, H. Matsui and T. Kanamori
    • Organizer
      PN&G 2014
    • Place of Presentation
      Tokyo
    • Year and Date
      2014-11-10 – 2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Microscale Cell Manipulation by Photodegradable Hydrogel2014

    • Author(s)
      Shinji Sugiura
    • Organizer
      20th Young Asian Biochemical Engineers’ Community Symposium (YABEC 2014)
    • Place of Presentation
      Chiayi, Taiwan
    • Year and Date
      2014-11-06 – 2014-11-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] 光分解性ハイドロゲル、培養器具、組織体形成方法及び細胞分離方法2016

    • Inventor(s)
      杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
    • Industrial Property Rights Holder
      杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-04-04
    • Related Report
      2016 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 光分解性ゲル、細胞培養器具、細胞配列・分別装置、細胞配列方法および細胞分別方法2016

    • Inventor(s)
      杉浦慎治、高木俊之、須丸公雄、金森敏幸
    • Industrial Property Rights Holder
      杉浦慎治、高木俊之、須丸公雄、金森敏幸
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-03-08
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] クリック架橋型光分解性ゲル2015

    • Inventor(s)
      杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
    • Industrial Property Rights Holder
      杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-077159
    • Filing Date
      2015-04-03
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 光分解性架橋剤、光分解性ゲル、細胞培養器具、細胞配列・分別装置、細胞配列方法、細胞分別方法、組織体形成方法および組織体2014

    • Inventor(s)
      杉浦慎治、高木俊之、柳川史樹、須丸公雄、金森敏幸
    • Industrial Property Rights Holder
      杉浦慎治、高木俊之、柳川史樹、須丸公雄、金森敏幸
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-05-13
    • Acquisition Date
      2016-12-16
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 光分解性架橋剤、光分解性ゲル、細胞培養器具、細胞配列・分別装置、細胞配列方法、細胞分別方法、組織体形成方法および組織体2014

    • Inventor(s)
      杉浦慎治、高木俊之、柳川史樹、須丸公雄、金森敏幸
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-05-13
    • Related Report
      2014 Annual Research Report
    • Overseas

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Published: 2014-04-04   Modified: 2019-03-29  

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