Development of 3D Imaging Cell Sorting Method

• Project/Area Number: 26709064
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Shinji Sugiura 国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (10399496)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 細胞分離 / ハイドロゲル / 癌 / 細胞培養 / 包埋培養 / 画像解析 / 生体材料 / 細胞 / バイオ関連機器

Outline of Final Research Achievements

In a conventional cell separation method such as a flow cytometer, cells are separated using a surface antigen as a marker. On the other hand, the markers for many heterogeneous cell populations such as cancer tissues and stem cell culture systems have been undefined yet, and it is expected to develop a method for separating cells by separately analyzing cell functions for each cell. In this study, we developed the "3D imaging cell sorting method" which separates cells using the photodegradable hydrogel using cell morphology as an indicator for cellular function under three dimensional culture.

Research Products

• [Journal Article] がんの多様性 (2016)
  • Author(s): 田村 磨聖、杉浦 慎治
  • Journal Title: 生物工学会誌 Volume: 94 Pages: 82-82
• [Journal Article] Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment (2015)
  • Author(s): Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
  • Journal Title: Scientific reports Volume: 5 Issue: 1 Pages: 15060-15060
  • DOI: 10.1038/srep15060
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 光分解性ゲルを用いたがん細胞選抜システムの開発 (2017)
  • Author(s): 杉浦慎治
  • Organizer: CBI学会2017年大会
• [Presentation] ハイドロゲル包埋培養系から生きたまま細胞を選抜する自動細胞分離装置の開発 (2017)
  • Author(s): 杉浦慎治、田村磨聖、山平真也、佐藤 琢、高木 俊之、須丸 公雄、金森 敏幸、渋田真結、 蟹江 慧、加藤竜司、柳沢真澄、松井裕史
  • Organizer: 化学工学会第49回秋季大会
• [Presentation] Optical cell manipulation using biomaterial-based photodegradable hydrogels (2016)
  • Author(s): Shinji Sugiura
  • Organizer: Fall Symposium 2016 of KIChE
  • Place of Presentation: Korea University, Daejon, Korea
  • Year and Date: 2016-10-19
  • Int'l Joint Research / Invited
• [Presentation] Development of an optical cell separation based on a cellular shape using click-crosslinkable and photodegradable gelatin hydrogels (2016)
  • Author(s): Masato Tamura, Shinji Sugiura, Taku Satoh, Toshiyuki Takagi, Toshiyuki Kanamori, Mayu Shibuta, Kei Kanie, Ryuji Kato, Hirofumi Matsui, Masumi Yanagisawa
  • Organizer: The Eighth International Conference on Microtechnologies in Medicine and Biology (MMB 2016)
  • Place of Presentation: Seoul National University、Seoul、韓国
  • Year and Date: 2016-04-20
  • Int'l Joint Research
• [Presentation] 『高スループット細胞分離技術を用いた「がん細胞薬剤感受性検査装置」の事業化』 (2016)
  • Author(s): 杉浦慎治
  • Organizer: つくばプロモーションイベント「健康長寿 Biz-seeds」
  • Place of Presentation: 常陽銀行、つくば
  • Year and Date: 2016-03-11
• [Presentation] 光分解性ゲルを用いた細胞操作技術の開発 (2016)
  • Author(s): 杉浦 慎治
  • Organizer: Bio Japan2016
  • Place of Presentation: パシフィコ横浜、横浜
  • Invited
• [Presentation] Development of an optical cell separation based on a cellular shape using click-crosslinkable and photodegradable gelatin hydrogels (2016)
  • Author(s): Masato Tamura, Shinji Sugiura, Taku Satoh, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori, Mayu Shibuta, Kei Kanie, Ryuji Kato, Hirofumi Matsui, Masumi Yanagisawa
  • Organizer: MMB 2016
  • Place of Presentation: Seoul University, Seoul, Korea
  • Int'l Joint Research
• [Presentation] Click-crosslinkable and photodegradable gelatin hydrogels for cell separation (2016)
  • Author(s): Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori,
  • Organizer: ICBS2016
  • Place of Presentation: 東京大学、東京
  • Int'l Joint Research
• [Presentation] Micropatterned Three-Dimensional Culture in Click-Crosslinkable and Photodegradable Gelatin Hydrogels (2015)
  • Author(s): Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru & Toshiyuki Kanamori
  • Organizer: 26th 2015 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: Nagoya University、Nagoya
  • Year and Date: 2015-11-23
  • Int'l Joint Research
• [Presentation] Optical cell seraration from 3D culture environment in photodegradable hydrogels for pure culture techniques (2014)
  • Author(s): M. Tamura, F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, H. Matsui and T. Kanamori
  • Organizer: EMBS Micro and Nanotechnology in Medicine Conference
  • Place of Presentation: Oahu
  • Year and Date: 2014-12-08 – 2014-12-12
• [Presentation] Photodegradable hydrogels for optical cell separation from three - dimensional environment (2014)
  • Author(s): M. Tamura, F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, H. Matsui and T. Kanamori
  • Organizer: The 10th SPSJ International Polymer Conference (IPC 2014)
  • Place of Presentation: Tsukuba
  • Year and Date: 2014-12-02 – 2014-12-05
• [Presentation] Optical cell separation with photodegradable hydrogels from three- dimensional environment for pure culture techniques (2014)
  • Author(s): M. Tamura, F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, H. Matsui and T. Kanamori
  • Organizer: PN&G 2014
  • Place of Presentation: Tokyo
  • Year and Date: 2014-11-10 – 2014-11-14
• [Presentation] Microscale Cell Manipulation by Photodegradable Hydrogel (2014)
  • Author(s): Shinji Sugiura
  • Organizer: 20th Young Asian Biochemical Engineers’ Community Symposium (YABEC 2014)
  • Place of Presentation: Chiayi, Taiwan
  • Year and Date: 2014-11-06 – 2014-11-08
  • Invited
• [Patent(Industrial Property Rights)] 光分解性ハイドロゲル、培養器具、組織体形成方法及び細胞分離方法 (2016)
  • Inventor(s): 杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
  • Industrial Property Rights Holder: 杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-04-04
  • Overseas
• [Patent(Industrial Property Rights)] 光分解性ゲル、細胞培養器具、細胞配列・分別装置、細胞配列方法および細胞分別方法 (2016)
  • Inventor(s): 杉浦慎治、高木俊之、須丸公雄、金森敏幸
  • Industrial Property Rights Holder: 杉浦慎治、高木俊之、須丸公雄、金森敏幸
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-03-08
  • Overseas
• [Patent(Industrial Property Rights)] クリック架橋型光分解性ゲル (2015)
  • Inventor(s): 杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
  • Industrial Property Rights Holder: 杉浦慎治、田村磨聖、高木俊之、須丸公雄、金森敏幸、柳川史樹
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-077159
  • Filing Date: 2015-04-03
• [Patent(Industrial Property Rights)] 光分解性架橋剤、光分解性ゲル、細胞培養器具、細胞配列・分別装置、細胞配列方法、細胞分別方法、組織体形成方法および組織体 (2014)
  • Inventor(s): 杉浦慎治、高木俊之、柳川史樹、須丸公雄、金森敏幸
  • Industrial Property Rights Holder: 杉浦慎治、高木俊之、柳川史樹、須丸公雄、金森敏幸
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-13
  • Acquisition Date: 2016-12-16
• [Patent(Industrial Property Rights)] 光分解性架橋剤、光分解性ゲル、細胞培養器具、細胞配列・分別装置、細胞配列方法、細胞分別方法、組織体形成方法および組織体 (2014)
  • Inventor(s): 杉浦慎治、高木俊之、柳川史樹、須丸公雄、金森敏幸
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-13
  • Overseas