Mechanisms for boron sensing by borate transceptors

• Project/Area Number: 26712007
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Osaka Prefecture University (2016-2017); Hokkaido University (2014-2015)
• Principal Investigator: TAKANO Junpei 大阪府立大学, 生命環境科学研究科, 教授 (70532472)
• Research Collaborator: YOSHINARI Akira ; WANG Sheliang
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 植物栄養 / センシング / エンドサイトーシス / ホウ素 / 細胞内輸送 / タンパク質分解 / 植物 / 発現制御 / 輸送体

Outline of Final Research Achievements

Plants sense mineral concentrations in soils or tissues and regulate membrane transport proteins to response to environmental changes. Boron is an essential element but is toxic when accumulated at high levels. Arabidopsis BOR1 is an borate exporter at the plasma membrane and responsible for boron transport toward root stele. BOR1 is transferred from the plasma membrane to the vacuole for degradation upon high-B supply to avoid boron toxicity. Our results in this study suggested that BOR1 functions as a borate/boric acid transceptor (transporter and receptor) regulating own accumulation in the plasma membrane to maintain boron homeostasis.

Research Products

• [Journal Article] Boron Uptake Assay in Xenopus laevis Oocytes (2018)
  • Author(s): Wang, S., Mitani-Ueno, N., Takano, J.
  • Journal Title: bio-protocol Volume: 8 Issue: 5 Pages: 5-5
  • DOI: 10.21769/bioprotoc.2755
  • Peer Reviewed / Open Access
• [Journal Article] Insights into the mechanisms underlying boron homeostasis in plants. (2017)
  • Author(s): Yoshinari, A., and ＊Takano, J.
  • Journal Title: Frontiers in Plant Science Volume: 17 Pages: 1-1
  • DOI: 10.3389/fpls.2017.01951
  • Peer Reviewed / Open Access
• [Journal Article] Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots. (2017)
  • Author(s): Wang S, Yoshinari A, Shimada T, Hara-Nishimura I, Mitani-Ueno N, Ma JF, Naito S, Takano J.
  • Journal Title: Plant Cell Volume: 印刷中 Issue: 4 Pages: 824-842
  • DOI: 10.1105/tpc.16.00825
  • Peer Reviewed
• [Journal Article] DRP1-dependent Endocytosis Is Essential for Polar Localization and Boron-induced Degradation of the Borate Transporter BOR1 in Arabidopsis thaliana (2016)
  • Author(s): Yoshinari, A., Fujimoto, M., Ueda, T., Inada, N., Naito, S. and Takano, J.
  • Journal Title: Plant Cell Phys. Volume: 57 Issue: 9 Pages: 1985-2000
  • DOI: 10.1093/pcp/pcw121
  • Peer Reviewed
• [Journal Article] Tolerance to Excess-Boron Conditions Acquired by Stabilization of a BOR1 Variant with Weak Polarity in Arabidopsis (2016)
  • Author(s): Shinji Wakuta; Teppei Fujikawa; Satoshi Naito; Junpei Takano
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 4
  • DOI: 10.3389/fcell.2016.00004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation (2016)
  • Author(s): Marie Barberon; Joop Engelbertus Martinus Vermeer; Damien De Bellis; Peng Wang; Sadaf Naseer; Tonni Grube Andersen; Bruno Martin Humbel; Christiane Nawrath; Junpei Takano; David Edward Salt; Niko Geldner
  • Journal Title: Cell Volume: 164 Issue: 3 Pages: 447-459
  • DOI: 10.1016/j.cell.2015.12.021
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells (2015)
  • Author(s): Sheliang Wang; Toshiaki Ito; Masataka Uehara; Satoshi Naito; Junpei Takano
  • Journal Title: Journal of Plant Research Volume: 128 Issue: 5 Pages: 863-873
  • DOI: 10.1007/s10265-015-0737-4
  • NAID: 120005906925
  • Peer Reviewed
• [Journal Article] Evolutionary Divergence of Plant Borate Exporters and Critical Amino Acid Residues for the Polar Localization and Boron-dependent Vacuolar Sorting of AtBOR1. (2015)
  • Author(s): Wakuta, S., Mineta,k., Amano, T., Toyoda,A., Fujiwara,T., Naito,S., and Takano, J.
  • Journal Title: Plant Cell Physiology Volume: 56 Issue: 5 Pages: 852-862
  • DOI: 10.1093/pcp/pcv011
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of Endocytosis and Ubiquitination of the BOR1 Transporter. (2014)
  • Author(s): Kasai, K., Takano, J., and Fujiwara, T.
  • Journal Title: Method in Molecular Biology Volume: 1209 Pages: 203-217
  • DOI: 10.1007/978-1-4939-1420-3_16
  • ISBN: 9781493914197, 9781493914203
  • Peer Reviewed / Open Access
• [Journal Article] A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. (2014)
  • Author(s): Pfister, A., Barberon, M., Alassimone, J., Kalmbach, L., Lee, Y., Vermeer, J. E., Yamazaki, M., Li, G., Maurel, C., Takano, J., Kamiya, T., Salt, D. E., Roppolo, D., and Geldner, D.
  • Journal Title: eLife Volume: 3 Pages: 1-20
  • DOI: 10.7554/elife.03115
  • Peer Reviewed / Open Access
• [Journal Article] Improved tolerance to boron deficiency by enhanced expression of the boron transporter BOR2. (2014)
  • Author(s): Takada, S., Miwa, K., Omori, H., Fujiwara, T., Naito, S., and Takano, J.
  • Journal Title: Soil Science and Plant Nutrition Volume: 60 Issue: 3 Pages: 341-348
  • DOI: 10.1080/00380768.2014.881705
  • Peer Reviewed / Open Access
• [Journal Article] OsNIP3;1, a rice boric acid channel, regulates boron distribution and is essential for growth under boron-deficient conditions. (2014)
  • Author(s): Hanaoka, H., Uraguchi, S., Takano, J., Tanaka, M. and Fujiwara, T.
  • Journal Title: The Plant Journal Volume: 78 Issue: 5 Pages: 890-902
  • DOI: 10.1111/tpj.12511
  • Peer Reviewed / Open Access
• [Journal Article] Identification and Characterization of an Arabidopsis Mutant with Altered Localization of NIP5;1, a Plasma Membrane Boric Acid Channel, Reveals the Requirement for D-Galactose in Endomembrane Organization. (2014)
  • Author(s): Uehara, M., Wang, S., Kamiya, T., Shigenobu, S., Yamaguchi, K., Fujiwara, T., Naito, S., and Takano, J.
  • Journal Title: Plant Cell Physiology Volume: 55 Issue: 4 Pages: 704-714
  • DOI: 10.1093/pcp/pct191
  • Peer Reviewed / Open Access
• [Presentation] A boron sensing mechanism regulating degradation of a borate transporter (2018)
  • Author(s): 高野順平
  • Organizer: 日本農芸化学会 名城大学
  • Invited
• [Presentation] The role of adaptor protein complex 4 (AP-4) in vacuolar targeting of a borate transporter AtBOR1 (2018)
  • Author(s): 細川卓也
  • Organizer: 第5９回日本植物生理学会年会 札幌
• [Presentation] ホウ酸トランスポーターの輸送活性に依存したユビキチン化と分解 (2018)
  • Author(s): 高野順平
  • Organizer: 第5９回日本植物生理学会年会 札幌
• [Presentation] Dissection of vacuolar trafficking pathway of the borate transporter BOR1 in Arabidopsis thaliana (2017)
  • Author(s): Akira Yoshinari, Korbei Barbara, Satoshi Naito, Junpei Takano
  • Organizer: 日本植物生理学会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] Polar Localization Of Boric acid/borate Transport Proteins (2017)
  • Author(s): 高野順平
  • Organizer: 日本植物生理学会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] Development and utilization of cytosolic boric acid sensors for boron-transport studies (2017)
  • Author(s): 高野順平
  • Organizer: Boron symposium, A satellite meeting of IPNC, Copenhagen
  • Int'l Joint Research
• [Presentation] ホウ酸トランスポーターBOR1におけるホウ酸輸送活性に依存した自己制御 (2017)
  • Author(s): 高野運平
  • Organizer: 日本土壌肥料学会仙台大会
• [Presentation] シロイヌナズナにおいてクラスリンアダプタータンパク質複合体AP2に依存したエンドサイトーシスはホウ酸輸送体の偏在とホウ素欠乏条件下での生育に必須である (2016)
  • Author(s): 吉成晃、西村浩二、天野太郎、藤本優、山岡尚平、嶋田知生、 上田貴志、西村いくこ、稲田のりこ、高野順平
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2016-09-22
• [Presentation] シロイヌナズナホウ酸チャネルNIP5;1の 細胞膜上偏在性は効率的なホウ酸輸送に貢献する (2016)
  • Author(s): Wang Sheliang, 永森彩奈, 三谷奈見季, 馬建鋒, 内藤哲, 高野順平（発表者)
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2016-09-22
• [Presentation] Polar Localization of a Boric Acid Channel NIP5;1 is Mediated by Phosphorylation and Required for Efficient Uptake of Boron in Arabidopsis thaliana (2016)
  • Author(s): Wang, S., Mitani-Ueno, N., Ma, JF., Naito, S., ○Takano, J.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県・盛岡市）
  • Year and Date: 2016-03-20
• [Presentation] BOR1 の細胞膜上での偏在にはクラスリンアダプター AP2 が必須であるものの、ホウ素に応答した分解に AP2 は不要である (2016)
  • Author(s): 吉成晃、藤本 優,天野 太郎,山岡 尚平,嶋田 知生,西村 いくこ,上田 貴志,内藤 哲,高野 順 平
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県・盛岡市）
  • Year and Date: 2016-03-20
• [Presentation] ホウ酸チャネルの ER exit に重要な因子 KNS3/SPOT1 の同定 (2016)
  • Author(s): 中村 俊介、上原 匡貴,竹村 駿介,石黒 澄衛,神谷 岳洋,藤原 徹,内藤 哲,高野 順平
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県・盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] ホウ酸輸送体 BOR1 の液胞輸送制御に重要なアミノ酸残基の同定と機能解析 (2016)
  • Author(s): 荻野 由香 、三浦 弓佳,和久田 真司,内藤 哲,高野 順平
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県・盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] ホウ酸センサーによる植物組織内ホウ酸分布の解析 (2015)
  • Author(s): 和久田真司、藤川哲平、内藤哲、○高野順平
  • Organizer: 土壌肥料学会京都大会
  • Place of Presentation: 京都大学 (京都府・京都市）
  • Year and Date: 2015-09-11
• [Presentation] シロイヌナズナ細胞膜局在型ホウ酸チャネルNIP5;1のER exitに必要な因子の同定 (2015)
  • Author(s): 中村俊介、上原 匡貴,内藤 哲,高野 順平
  • Organizer: 土壌肥料学会京都大会
  • Place of Presentation: 京都大学 (京都府・京都市）
  • Year and Date: 2015-09-11
• [Presentation] シロイヌナズナホウ酸輸送体BOR1によるホウ酸センシングの可能性 (2015)
  • Author(s): 荻野 由香、吉成 晃，和久田 真司，内藤 哲，高野 順平
  • Organizer: 土壌肥料学会京都大会
  • Place of Presentation: 京都大学 (京都府・京都市）
  • Year and Date: 2015-09-09
• [Presentation] Phosphorylation-mediated polar localization of a boric acid channel is required for efficient uptake of boron in Arabidopsis thaliana (2015)
  • Author(s): Wang, S., Mitani-Ueno, N., Ma, JF., Naito, S., Takano, J.
  • Organizer: 13th ICOBTE
  • Place of Presentation: 福岡国際会議場 (福岡県・福岡市）
  • Year and Date: 2015-07-15
  • Int'l Joint Research
• [Presentation] AP2-dependent and independent endocytosis distinctly regulate polar localization and degradation of a borate transporter BOR1 in Arabidopsis thaliana (2015)
  • Author(s): Akira Yoshinari, Tetsuya Kurata, Tomoaki Sakamoto, Satoshi Naito, Junpei Takano
  • Organizer: 13th ICOBTE
  • Place of Presentation: 福岡国際会議場 (福岡県・福岡市）
  • Year and Date: 2015-07-15
  • Int'l Joint Research
• [Presentation] Mechanisms for boron-dependent vacuolar trafficking of a boric acid/borate transporter AtBOR1 (2015)
  • Author(s): Y. Ogino, A.Yoshinari, S.Wakuta, S.Naito, and J.Takano
  • Organizer: 13th ICOBTE
  • Place of Presentation: 福岡国際会議場 (福岡県・福岡市）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] Polar localization and boron-dependent vacuolar trafficking of boric acid/borate transporters in Arabidopsis thaliana. (2014)
  • Author(s): Junpei Takano
  • Organizer: 内藤コンファレンス
  • Place of Presentation: ガトーキングダムサッポロ（札幌市）
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] 植物のホウ酸輸送体の細胞膜上極性局在とホウ酸に応答したエンドサイトーシス系分解のメカニズム (2014)
  • Author(s): 高野順平
  • Organizer: 日本農芸化学会北海道•東北支部 合同若手の会
  • Place of Presentation: 定山渓ビューホテル（札幌市）
  • Year and Date: 2014-09-24
  • Invited
• [Presentation] Toward understanding the mechanisms of boron sensing and intracellular trafficking of boric acid/borate transporters (2014)
  • Author(s): 高野順平
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京農工大学（東京都府中市）
  • Year and Date: 2014-09-11
• [Presentation] 植物におけるホウ酸トランスポーターBORの進化系統解析 (2014)
  • Author(s): 和久田真司、峯田克彦, 天野太郎, 井手康二, 内藤哲, 高野順平
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京農工大学（東京都府中市）
  • Year and Date: 2014-09-09
• [Presentation] シロイヌナズナのホウ酸輸送体BOR1の分解に必要な因子の同定 (2014)
  • Author(s): 吉成晃、坂本智昭、倉田哲也、内藤哲、高野順平
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京農工大学（東京都府中市）
  • Year and Date: 2014-09-09
• [Book] Plant Aquaporins (2017)
  • Author(s): Junpei Takano, Akira Yoshinari, and Doan-Trung Luu
  • Publisher: Springer
• [Book] Frontiers of Agricultural Science (2015)
  • Author(s): Junpei Takano 計64名
  • Total Pages: 277
  • Publisher: SHOUKADOH
• [Remarks] 植物栽培生理学研究グループ
  • URL: http://saibaiseirigaku.wixsite.com/crop-ecophysiology
• [Remarks] 分子生物学研究室
  • URL: http://www.agr.hokudai.ac.jp/arabi/
• [Remarks] HOKUDAI NEXT
  • URL: http://or.research.hokudai.ac.jp/next/
• [Remarks] 知のフロンティア 北海道大学の研究は、いま
  • URL: http://www.hokudai.ac.jp/bureau/nyu/frontier/01.html
• [Remarks] Hokkaido University Magazine 2013
  • URL: http://www.oia.hokudai.ac.jp/wp-content/uploads/2013/03/magazine2013english.pdf