Study on ER stress response involved in toxicity of acrylamide by using zebrafish
| Project/Area Number |
26740025
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Komoike Yuta 東京女子医科大学, 医学部, 講師 (70386556)
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| Research Collaborator |
MATSUMURA Kenichi 東京女子医科大学, 医学部, 実験補助員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ゼブラフィッシュ / 環境毒性学 / 小胞体ストレス応答 / アクリルアミド |
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| Outline of Final Research Achievements |
Acrylamide has recently been recognized as an immediate hazardous chemical worldwide. In this study, this applicant focused on the toxicity of acrylamide, especially on the mechanisms underlying expression of acrylamide-induced neurotoxicity. The applicant revealed that exposure of human neuroblastoma cells to acrylamide increases accumulation of reactive oxygen species (ROS) and these ROS evoke endoplasmic reticulum (ER) stress response followed by cell death including apoptosis. The applicant also showed that exposure of zebrafish larvae to acrylamide induces expression of the gene encoding ER stress response-dependent proapoptotic factor in the brain, apoptotic death of the brain cells, and dramatic disruption of the brain structure. These findings were reported in an original article and domestic conferences four times.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Single Nucleotide Variations in CLCN6 Identified in Patients with Benign Partial Epilepsies in Infancy and/or Febrile Seizures.2015
Author(s)
Yamamoto T, Shimojima K, Sangu N, Komoike Y, Ishii A, Abe S, Yamashita S, Imai K, Kubota T, Fukasawa T, Okanishi T, Enoki H, Tanabe T, Saito A, Furukawa T, Shimizu T, Milligan CJ, Petrou S, Heron SE, Dibbens LM, Hirose S, Okumura A.
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Journal Title
PLoS ONE
Volume: 10(3)
Issue: 3
Pages: e0118946-e0118946
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Novel compound heterozygous mutations of POLR3A revealed by whole-exome sequencing in a patient with hypomyelination2014
Author(s)
Shimojima, K., Shimada, S., Tamasaki, A., Akaboshi, S., Komoike, Y., Saito, A., Furukawa, T., Yamamoto, T.
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Journal Title
Brain Dev.
Volume: 36
Issue: 4
Pages: 315-321
DOI
Related Report
Peer Reviewed
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[Presentation] Single nucleotide variation in CLCN6 identified in patients with benign partial epilepsies in infancy and/or febrile seizures2016
Author(s)
Yamamoto T, Shimojima K, Komoike Y, Ishii A, Abe S, Yamashita S, Imai K, Kubota T, Fukasawa T, Okanishi T, Enoki H, Tanabe T, Saito A, Furukawa T, Shimizu T, Milligan CJ, Petrou S, Heron SE, Dibbens LM, Hirose S, Okumura A
Organizer
The 13th International Congress of Human Genetics
Place of Presentation
京都国際会議場（京都府京都市）
Year and Date
2016-04-03
Related Report
Int'l Joint Research
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