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Evaluation of the genotoxicity of thermally treated soils using the electrochemical genotoxicity assay based on a SOS/umu test

Research Project

Project/Area Number 26740042
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental risk control and evaluation
Research InstitutionUniversity of Toyama

Principal Investigator

Sazawa Kazuto  富山大学, 大学院理工学研究部(理学), 助教 (80727016)

Co-Investigator(Renkei-kenkyūsha) WADA Naoya  富山大学, 研究推進機構極東地域研究センター, 教授 (40272893)
KURAMITZ Hideki  富山大学, 大学院理工学研究部(理学), 教授 (70397165)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords土壌有機成分 / 森林火災 / 多環芳香族炭化水素 / 遺伝毒性 / 土壌 / 電気化学 / 火災
Outline of Final Research Achievements

The effect of forest fire can be seen in the physical, chemical and biological properties of soil. Polycyclic aromatic hydrocarbons (PAHs) are produced by the incomplete combustion of soil organic matter, and they induce DNA damage in bacterial cell. The purpose of this study is to evaluate the genotoxicity of thermally treated soils using a new umu test based on hydrodynamic electrochemical detection.
It was found that the genotoxicity of soil drastically increase when samples were heated over 350 ℃. The intensity of genotoxicity showed a good liner correlation with the concentration of three- and four-rings PAHs and Δb* values of thermally treated samples. The change of Δb* value is strongly related to the dehydration and decarboxylation of soil organic matter. The result obtained from this study concluded that the soil with a high organic matter content such as peat soil indicate high genotoxicity risk.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Hydrodynamic voltammetry as a rapid and simple method for evaluating soil enzyme activities2015

    • Author(s)
      Sazawa, K., Kuramitz, H.
    • Journal Title

      Sensors

      Volume: 15 Issue: 3 Pages: 5331-5343

    • DOI

      10.3390/s150305331

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 電気化学的遺伝毒性試験法を用いた加熱土壌の遺伝毒性評価と発現条件に関する研究2015

    • Author(s)
      佐澤和人, 和田直也, 倉光英樹
    • Organizer
      日本生態学会中部地区会2015年度大会
    • Place of Presentation
      高山市民文化会館
    • Year and Date
      2015-10-24
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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