| Project/Area Number |
26750145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TAMAI Miho 東京工業大学, 生命理工学研究科, 東工大特別研究員 (20619704)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | in vitro モデル / 肝組織 / 肝炎 / リンパ球 / ES/iPS細胞 / in vitro 肝炎モデル / 肝障害 / 炎症反応 / サイトカイン |
|---|
| Outline of Final Research Achievements |
Hepatocyte injury could be observed in the mouse ES cell-derived hepatic tissue in vitro model after the addition of acetaminophen, APAP. This result was similar to the APAP-induced liver injury in a dependent manner on the hepatocyte-specific metabolism in mice. So, in order to investigate contribution of immune cells in the APAP-induced liver injury, lymphocytes which were prepared from syngeneic mouse spleen were co-cultured in the hepatic tissue in vitro model. The injury level was enhanced in the in vitro model co-cultured with the lymphocytes compared to that without ones. As mentioned above, besides direct drug-induced cell death, it could be indicated that the hepatic tissue in vitro model can become the injury by associating with inflammatory cytokines.
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