• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a method to identify the specific affinity of carbohydrates for the cancer tissue

Research Project

Project/Area Number 26750364
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomolecular chemistry
Research InstitutionOsaka University

Principal Investigator

Shimoyama Atsushi  大阪大学, 理学(系)研究科(研究院), 助教 (90625055)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞内共生菌 / リポ多糖 / TLR4-MD2 / 腫瘍ターゲティング / 免疫制御 / リピドA / 自然免疫 / 共生菌 / TLR4 / 糖化学 / 糖鎖 / がん / 免疫
Outline of Final Research Achievements

To accomplish the specific cell recognition system by using the interaction between carbohydrate molecules and their receptor, carbohydrate molecules having specific interaction with cancer tissue but having no toxicity were investigated.
Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria, and is recognized by TLR4-MD2 complex to induce potent immunostimulating activities. Recent findings show that TLR4 are expressed not only on immune cells but also on cancer cells. Therefore, LPS has great potential as an immunomodulating molecule including the capability of cancer clustering. However, LPS generally shows cytotoxicity because of its potent inflammatory activities. To reveal the non-toxic TLR4 ligands, we focused on intracellular symbiotic bacterial LPS. The isolation method of the intracellular symbiotic bacterial LPS from freeze-dried cell was confirmed and the chemical structure of intracellular symbiotic bacterial LPS was determined.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (29 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (22 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Napoli Federico II(イタリア)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Effective Synthesis of Oligosaccharide under Microfluidic Conditions2015

    • Author(s)
      Fukase, K.; Shimoyama, A.; Manabe, Y.
    • Journal Title

      Journal of Synthetic Organic Chemistry, Japan

      Volume: 73 Issue: 5 Pages: 452-459

    • DOI

      10.5059/yukigoseikyokaishi.73.452

    • NAID

      130005077685

    • ISSN
      0037-9980, 1883-6526
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 分子イメージングによる糖鎖複合体の動態解析2015

    • Author(s)
      深瀬浩一、田中克典、下山敦史
    • Journal Title

      日本分子イメージング学会機関誌 JSMI report

      Volume: 8(2) Pages: 18-25

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Biological Activity of Inositol Phospholipid as NKT Cell Modulator2015

    • Author(s)
      Aiba, T., Suehara, S., Bernin, H., Lotter, H., Inuki, S., Fukase, K., Fujimoto, Y.
    • Journal Title

      Glycoconjugate J.

      Volume: 32 Issue: 5 Pages: 301-301

    • DOI

      10.1007/s10719-015-9596-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bioorthogonal Reactions for Biomolecular Functionalization and Imaging2014

    • Author(s)
      Atsushi Shimoyama
    • Journal Title

      Journal of Synthetic Organic Chemistry, Japan

      Volume: 72 Issue: 3 Pages: 303-304

    • DOI

      10.5059/yukigoseikyokaishi.72.303

    • NAID

      130003395396

    • ISSN
      0037-9980, 1883-6526
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 共役イミンの[4+4]環化付加反応を用いた不飽和アルデヒド検出法の開発2016

    • Author(s)
      土田紘也、下山敦史、田中克典、深瀬浩一
    • Organizer
      日本ケミカルバイオロジー学会 第11回年会
    • Place of Presentation
      京都テルサ、京都
    • Year and Date
      2016-06-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホスファチジルイノシトールの合成とそのNKT細胞刺激活性2016

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      日本ケミカルバイオロジー学会 第11回年会
    • Place of Presentation
      京都テルサ、京都
    • Year and Date
      2016-06-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chemical Synthesis of Immunomodulative Lipopolysaccharide Partial Structures2016

    • Author(s)
      A. Shimoyama, Y. Fujimoto, K. Fukase
    • Organizer
      The 12th International Symposium on Organic Reactions (ISOR12)
    • Place of Presentation
      Kyoto Terrsa, Kyoto, Japan
    • Year and Date
      2016-04-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光親和性標識化ペプチドグリカンフラグメントの合成と機能2016

    • Author(s)
      富澤一美、下山敦史、王倩倩、藤本ゆかり、深瀬浩一
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス、京都
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 共役イミンの二量化反応を用いた不飽和アルデヒド検出法の開発2016

    • Author(s)
      土田紘也、下山敦史、田中克典、深瀬浩一
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス、京都
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 内因性CD1dリガンドとしてのホスファチジルイノシトールの合成とその生理活性2016

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス、京都
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 免疫調節作用を有するリポ多糖部分構造の化学合成とその機能解析2016

    • Author(s)
      下山敦史、藤本ゆかり、深瀬浩一
    • Organizer
      JST-ERATO村田脂質活性構造プロジェクト最終成果報告会
    • Place of Presentation
      大阪大学会館、大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 内因性CD1dリガンドとしてのホスファチジルイノシトールの化学合成とその生物活性2016

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      JST-ERATO村田脂質活性構造プロジェクト最終成果報告会
    • Place of Presentation
      大阪大学会館、大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホスファチジルイノシトールの合成とその生物活性2016

    • Author(s)
      下山敦史、中川 翔、藤本ゆかり、深瀬浩一
    • Organizer
      第19回生理活性をあまり意図しない自由な天然物合成勉強会
    • Place of Presentation
      アワーズイン阪急、東京
    • Year and Date
      2016-02-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] Synthesis and biological activity of phosphatidylinositol for the elucidation of NKT cell pre-activation mechanism2015

    • Author(s)
      S. Nakagawa, A. Shimoyama, T. Aiba, Y. Fujimoto, K. Fukase
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Kinetic control Fischer glycosylation using acidic silica gel under fluidic conditions2015

    • Author(s)
      S. Masui, Y. Manabe, A. Shimoyama, T. Fukuyama, I. Ryu, K. Fukase
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of LPS/lipid A from Alcaligenes sp.2015

    • Author(s)
      K. Mizote, A. Shimoyama, N. Shibata, F. D. Lorenzo, Y. Fujimoto, A. Molinaro, J. Kunisawa, H. Kiyono, K. Fukase
    • Organizer
      7th Asian Community of Glycoscience and Glycotechnology (ACGG)
    • Place of Presentation
      Hotel Taikanso, Matsushima, Miyagi
    • Year and Date
      2015-11-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Synthesis and Bio-functional Studies of Immunomodulating LPS Partial Structures2015

    • Author(s)
      A. Shimoyama, K. Mizote, Y. Fujimoto, K. Fukase
    • Organizer
      the 13th International Kyoto Conference on New Aspects of Organic Chemistry (IKCOC-13)
    • Place of Presentation
      Rihga Royal Hotel KYOTO, JAPAN
    • Year and Date
      2015-11-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 内因性CD1dリガンドの探索を目指したホスファチジルイノシトールの合成とその生理活性2015

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      第5回CSJ化学フェスタ
    • Place of Presentation
      タワーホール船堀、東京
    • Year and Date
      2015-10-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Synthesis of Porphyromonas gingivalis Lipid A2015

    • Author(s)
      下山敦史、藤本ゆかり、深瀬浩一
    • Organizer
      第18回生理活性をあまり意図しない自由な天然物合成勉強会
    • Place of Presentation
      ホテル新大阪、大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Synthesis and Biofunctional Study of Immunomodulative Lipopolysaccharide Partial Structures2015

    • Author(s)
      A. Shimoyama, K. Mizote, Y. Fujimoto, K. Fukase
    • Organizer
      23rd International Symposium on Glycoconjugates (Glyco23)
    • Place of Presentation
      Hotel Le Meridien Lav, Split, Croatia
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 免疫調節作用を有するリポ多糖部分構造の化学合成とその機能解析2015

    • Author(s)
      下山敦史、藤本ゆかり、深瀬浩一
    • Organizer
      ERATO村田脂質活性構造プロジェクト夏のセミナー
    • Place of Presentation
      エクシブ琵琶湖、滋賀
    • Year and Date
      2015-08-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] NKT細胞の前活性化機構解明を目指したホスファチジルイノシトールの合成とその生理活性2015

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      第34回日本糖質学会年会
    • Place of Presentation
      東京大学 安田講堂、東京
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホスファチジルイノシトールの合成とその生理活性2015

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      FCCAグライコサイエンス若手フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2015-07-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] NKT細胞の活性化機構解明を目指したホスファチジルイノシトールの合成とその生理活性2015

    • Author(s)
      中川翔、下山敦史、相羽俊彦、藤本ゆかり、深瀬浩一
    • Organizer
      第50回天然物化学談話会
    • Place of Presentation
      仙台
    • Year and Date
      2015-07-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 酸性シリカゲルを用いたフロー系でのフィッシャーグリコシル化2015

    • Author(s)
      増井誠二、真鍋良幸、下山敦史、福山高英、柳日馨、深瀬浩一
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学、理工学部船橋キャンパス
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 糖質を用いたがん組織のイメージング手法の開発2015

    • Author(s)
      下山敦史
    • Organizer
      第17回生理活性をあまり意図しない自由な天然物合成勉強会
    • Place of Presentation
      阪急大井町ガーデン
    • Year and Date
      2015-02-28 – 2015-03-01
    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪大学 深瀬研究室 ホームページ

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/fukase/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 大阪大学 大学院理学研究科 化学専攻 深瀬研究室

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/fukase/index.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi