| Project/Area Number |
26750365
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Ehime University |
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Principal Investigator |
Hiroyuki Takeda 愛媛大学, プロテオサイエンスセンター, 准教授 (40609393)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | モノクローナル抗体 / GPCR / 抗原 / 無細胞タンパク質合成 / 味覚受容体 / リポソーム / 膜タンパク質 / 相互作用 / コムギ無細胞系 / 脂質 / コムギ無細胞合成 / プロテオリポソーム / 抗体 |
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| Outline of Final Research Achievements |
T1R family GPCR is sweet/umami receptor. Overexpression system and specific antibodies of T1R family were not established. In this study, we synthesized T1R1 and T1R3 using cell-free membrane protein expression system. Using cell-free synthesized T1Rs, we developed specific monoclonal antibodies. In addition to anti-T1R antibody, we also obtained monoclonal antibody against asolectin lipid. Anti-asolectin antibody bound to asolectin liposome, however it did not EggPC liposome. We developed an assay method to detect interaction between liposomes. Monoclonal antibodies and assay methods developed in this study may contribute to functional analysis of membrane proteins including taste receptors.
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