Construction of amylose hybrid polymer library for next-generation chaperone system.

• Project/Area Number: 26810113
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Polymer/Textile materials
• Research Institution: Kyoto University
• Principal Investigator: Nishimura Tomoki 京都大学, 工学(系)研究科(研究院), 研究員 (60648070)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アミロース / 酵素重合 / シャペロン / 両親媒性ポリマー / 糖鎖

Outline of Final Research Achievements

We prepared a library of amphiphilic amylose-based star copolymers using a copper catalyzed click reaction. We then evaluated chaperon function of a series of these molecules for heat denatured carbonic anhydrate and optimized chemical structure for chaperon functioning polymer. We also synthesized a novel light-sensitive nanogel using cholesterol modified with an ortho-nitrobenzyl unit on the hydroxyl group of pullulan. The use of light-sensitive components makes it possible to create patterned nanogel films that can immobilize proteins. The preparation of patterned materials is important for the construction of micro- or nanodevices such as MEMS and biomolecular chips.

Research Products

• [Journal Article] A light sensitive self-assembled nanogel as a tecton for protein patterning materials (2016)
  • Author(s): T. Nishimura, M. Takara, S, Mukai, S, Sawada, Y. Sasaki, K. Akiyoshi
  • Journal Title: Chemical communications Volume: 52 Pages: 1222-1225
  • Peer Reviewed / Open Access
• [Journal Article] Amylose-Based Cationic Star Polymers for siRNA Delivery (2015)
  • Author(s): T. Nishimura, K. Umezaki, S, Mukai, S, Sawada, K. Akiyoshi
  • Journal Title: BioMed Research International Volume: 2015 Pages: 1-5
  • DOI: 10.1155/2015/962941
  • NAID: 120005745655
  • Peer Reviewed / Open Access
• [Journal Article] Long-Lasting and Efficient Tumor Imaging Using a High Relaxivity Polysaccharide Nanogel Magnetic Resonance Imaging Contrast Agent (2015)
  • Author(s): M. Chan, J. Lux, T. Nishimura, K Akiyoshi, and A. Almutairi
  • Journal Title: Biomacromolecules Volume: 16 Pages: 2964-2971
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Glyco Star Polymers as Helical Multivalent Host and Biofunctional Nano-Platform (2015)
  • Author(s): Tomoki Nishimura, Sad-atsu Mukai, Shinichi Sawada, Kazunari Akiyoshi
  • Journal Title: ACS Macro Letters Volume: 4 Issue: 4 Pages: 367-371
  • DOI: 10.1021/acsmacrolett.5b00049
  • Peer Reviewed
• [Presentation] 自己組織化グライコカプセルの設計と人工ナノオルガネラへの応用 (2016)
  • Author(s): 西村 智貴、向井 貞篤、澤田 晋一、佐々木 善浩、秋吉 一成
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学、京都
  • Year and Date: 2016-03-24
• [Presentation] 物質透過性グライコカプセルの設計とナノバイオリアクターへの応用 (2016)
  • Author(s): 西村 智貴、河﨑 陸、向井 貞篤、澤田 晋一、佐々木 善浩、秋吉 一成
  • Organizer: 第25回インテリジェント材料/システムシンポジウム
  • Place of Presentation: 東京女子医科大学 先端生命医科学研究所 TWIns, 東京
  • Year and Date: 2016-01-08
• [Presentation] Amylose-Based Glyco Star Polymers: A Bio-functional Platform for Glyco-biomaterials (2015)
  • Author(s): Tomoki Nishimura, Sada-atsu Mukai, Shinichi Sawada, Kazunari Akiyoshi
  • Organizer: The 2015 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 糖鎖プライマー酵素重合によるグライコスターポリマーの創製とバイオ応用 (2015)
  • Author(s): 西村智貴・向井貞篤・澤田晋一・秋吉一成
  • Organizer: 第34回日本糖質学会
  • Place of Presentation: 東京大学安田講堂、東京
  • Year and Date: 2015-07-31
• [Presentation] タンデム酵素重合法によるハイパーブランチ糖鎖ハイブリッドの創製 (2015)
  • Author(s): 西村智貴・向井貞篤・澤田晋一・秋吉一成
  • Organizer: 第64会高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター, 北海道
  • Year and Date: 2015-05-27
• [Presentation] アミロース工学：酵素重合法によるグライコハイブリッドポリマーの構築 (2014)
  • Author(s): 西村智貴・向井貞篤・澤田晋一・秋吉一成
  • Organizer: 第63回高分子討論会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2014-09-24 – 2014-09-26
• [Presentation] Amylose Based Glyco star copolymers: A platform for new glyco-biomaterials. (2014)
  • Author(s): Tomoki Nishimura, Sada-atsu Mukai, Shin-ichi Sawada, Kazunari Akiysohi
  • Organizer: The 41st Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hilton Chicago Hotel
  • Year and Date: 2014-07-12 – 2014-07-16
• [Presentation] 機能性アミロース-グライコスターポリマーの設計とバイオ応用 (2014)
  • Author(s): 西村智貴・向井貞篤・澤田晋一・秋吉一成
  • Organizer: 第63回高分子学会年次大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2014-05-28 – 2014-05-30
• [Book] 糖鎖の新機能開発・応用ハンドブック 創薬・医療から食品開発まで (2015)
  • Author(s): 西村 智貴、秋吉 一成
  • Total Pages: 5
  • Publisher: 株式会社 NTS
• [Patent(Industrial Property Rights)] 両親媒性ブロック共重合体、分子集合体及びその製造方法 並びにタンパク質の内包剤 (2015)
  • Inventor(s): 秋吉一成、西村智貴
  • Industrial Property Rights Holder: 秋吉一成、西村智貴
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-09-24