Molecular mechanisms governing glia-neuron interaction in the sensory system

• Project/Area Number: 26830049
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Osaka City University
• Principal Investigator: KAGE-NAKADAI Eriko 大阪市立大学, 大学院生活科学研究科, 准教授 (40453790)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: グリア細胞 / 線虫 / 浸透圧 / 感覚器 / グリア / コンディショナルノックアウト

Outline of Final Research Achievements

Glial cells control many aspects of the nervous system, including the regulation of survival, differentiation and interconnection of neurons, in both vertebrates and invertebrates. The Caenorhabditis elegans (C. elegans) amphid sensory organ is a simple model for analyzing glia or neuron-glia interactions. To better characterize glial development and function, we carried out RNA interference screening for transcription factors that regulate the expression of an amphid sheath glial cell marker and identified pros-1, which encodes a homeodomain transcription factor homologous to Drosophila prospero/mammalian Prox1, as a positive regulator. We demonstrated that the C. elegans PROS-1 is a transcriptional regulator in the glia but is involved not only in sensory behavior but also in sensory-mediated physiological tolerance.

Research Products

• [Journal Article] Sesamin extends lifespan through pathways related to dietary restriction in Caenorhabditis elegans (2018)
  • Author(s): Nakatani, Y., Yaguchi, Y., Komura, T., Nakadai, M., Terao, K., Kage-Nakadai, E., and Nishikawa, Y.
  • Journal Title: Eur. J. Nut. Volume: 57 Issue: 3 Pages: 1137-1146
  • DOI: 10.1007/s00394-017-1396-0
  • Peer Reviewed
• [Journal Article] Influence of lactic acid and post-treatment recovery time on the heat resistance of Listeria monocytogenes. (2017)
  • Author(s): Omori Y, Miake K, Nakamura H, Kage-Nakadai E, Nishikawa Y.
  • Journal Title: Int J Food Microbiol. Volume: 257 Pages: 10-18
  • DOI: 10.1016/j.ijfoodmicro.2017.06.008
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence of Diarrheagenic <i>Escherichia coli</i> in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan (2017)
  • Author(s): 95.Wang, L., Zhang, S., Zheng, D., Fujihara, S., Wakabayashi, A., Okahata, K., Suzuki, M., Saeki, A., Nakamura, H., Hara-Kudo, Y., Kage-Nakadai, E., and Nishikawa, Y.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 70 Issue: 4 Pages: 464-469
  • DOI: 10.7883/yoken.JJID.2016.486
  • NAID: 130005851923
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prevalence, antimicrobial resistance and multiple-locus variable-number tandem-repeat analysis profiles of diarrheagenic Escherichia coli isolated from different retail foods (2017)
  • Author(s): Wang Lili、Nakamura Hiromi、Kage-Nakadai Eriko、Hara-Kudo Yukiko、Nishikawa Yoshikazu
  • Journal Title: Int. J. Food Microbiol. Volume: 249 Pages: 44-52
  • DOI: 10.1016/j.ijfoodmicro.2017.03.003
  • NAID: 120006485147
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Caenorhabditis elegans homolog of Prox1/Prospero is expressed in the glia and is required for sensory behavior and cold tolerance. (2016)
  • Author(s): Kage-Nakadai E., Ohta, A., Ujisawa T., Sun S., Nishikawa Y., Kuhara A., Mitani S.
  • Journal Title: Genes to Cells Volume: 21(9) Issue: 9 Pages: 936-948
  • DOI: 10.1111/gtc.12394
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distinct roles of the two VPS33 proteins in the endolysosomal system in Caenorhabditis elegans (2016)
  • Author(s): Gengyo-Ando K, Kage-Nakadai E, Yoshina S, Otori M, Kagawa-Nagamura Y, Nakai J, Mitani S
  • Journal Title: Traffic Volume: 17 Issue: 11 Pages: 1197-1213
  • DOI: 10.1111/tra.12430
  • Peer Reviewed / Open Access
• [Journal Article] Curcumin improves tau-induced neuronal dysfunction of nematodes. (2016)
  • Author(s): Miyasaka, T., Xie, C., Yoshimura, S., Shinzaki, Y., Yoshina, S., Kage-Nakadai, E., Mitani, S., Ihara, Y.
  • Journal Title: Neurobiol. Aging Volume: 39 Pages: 69-81
  • DOI: 10.1016/j.neurobiolaging.2015.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Locus-specific integration of extrachromosomal transgenes in C. elegans with the CRISPR/Cas9 system (2016)
  • Author(s): Yoshina et al.
  • Journal Title: BBRep Volume: 5 Pages: 70-76
  • DOI: 10.1016/j.bbrep.2015.11.017
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of unstable pEntYN10 from enterotoxigenic Escherichia coli (ETEC) O169:H41 (2015)
  • Author(s): 102.Ban, E., Yoshida, Y., Wakushima, M., Wajima, T., Hamabata, T., Ichikawa, N., Abe, H., Horiguchi, Y., Hara-Kudo, Y., Kage-Nakadai, E., Yamamoto, T., Wada, T., and Nishikawa, Y.
  • Journal Title: Virulence Volume: 6 Issue: 8 Pages: 735-744
  • DOI: 10.1080/21505594.2015.1094606
  • Peer Reviewed / Open Access
• [Journal Article] The Tumor Suppressor BCL7B Functions in the Wnt Signaling Pathway. (2015)
  • Author(s): Uehara T, Kage-Nakadai E, Yoshina S, Imae R, Mitani S.
  • Journal Title: PLoS Genet. Volume: 11 Issue: 1 Pages: e1004921-e1004921
  • DOI: 10.1371/journal.pgen.1004921
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Conditional Knockout Toolkit for Caenorhabditis elegans Based on the Cre/loxP Recombination. (2014)
  • Author(s): Kage-Nakadai E, Imae R, Suehiro Y, Yoshina S, Hori S, Mitani S.
  • Journal Title: PLoS One. Volume: 9 Issue: 12 Pages: e114680-e114680
  • DOI: 10.1371/journal.pone.0114680
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans. (2014)
  • Author(s): Kage-Nakadai E, Imae R, Yoshina S, Mitani S
  • Journal Title: Methods Volume: Mar 12 Issue: 3 Pages: 91-7
  • DOI: 10.1016/j.ymeth.2014.02.036
  • Peer Reviewed / Open Access
• [Journal Article] Influence of oral supplementation with sesamin on longevity of Caenorhabditis elegans and the host defense. (2014)
  • Author(s): Yaguchi, Y., Komura, T., Kashima, N., Tamura, M., Kage-Nakadai, E., Saeki, S., Terao, K., Nishikawa, Y.
  • Journal Title: Eur. J. Nut. Volume: 53 Issue: 8 Pages: 1659-68
  • DOI: 10.1007/s00394-014-0671-6
  • NAID: 120006576122
  • Peer Reviewed / Open Access
• [Presentation] Influence of Propionibacterium acnes on host defense of Caenorhabditis elegans, (2017)
  • Author(s): Yumi Hamazaki, Satomi Kamimura, Satoshi Inada, Simo Sun, Tomomi Komura, Shuta Tomida, Yoshikazu Nishikawa, Eriko Kage-Nakadai
  • Organizer: The 16th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] Diffusely adherent Escherichia coli (DAEC) strains isolated from healthy carriers inhibit IL-8 secretion of epithelial cells by the type VI secretion system (T6SS) (2017)
  • Author(s): Narumi Takeuchi, Yoshihiko Tanimoto, Sayaka Tamai, Saki Yanagida, Yoshihiro Yamaguchi, Eriko Kage-Nakadai, Yoshikazu Nishikawa
  • Organizer: The 16th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] Adhesion of human enterotoxigenic Escherichia coli (ETEC) O169:H41 to porcine intestinal epithelial cells by the novel colonization factor (2017)
  • Author(s): Omori, Y., Zheng, D., Ban, E., Kage-Nakadai, E., Tachibana, T., Wada, T., Hara-Kudo, Y., and Nishikawa, Y
  • Organizer: Vaccines for Enteric Diseases
  • Int'l Joint Research
• [Presentation] 線虫C.elegansを用いた食品に含まれる苦味物質に対する応答のメカニズム解明 (2017)
  • Author(s): 飯田千晴、西川禎一、中台（鹿毛）枝里子
  • Organizer: 第71回日本栄養・食糧学会大会
• [Presentation] 線虫におけるビフィズス菌(Bifidobacterium infantis)への低嗜好性に関わるニューロンおよび分子メカニズム (2017)
  • Author(s): 孫 思墨、西川 禎一、中台 枝里子
  • Organizer: 第56回日本栄養・食糧学会近畿支部大会
• [Presentation] 線虫 C.elegans を用いた食品中の苦味物質に対する生体応答の解析 (2017)
  • Author(s): 飯田千晴、孫思墨、西川禎一、中台(鹿毛)枝里子
  • Organizer: 第56回日本栄養・食糧学会近畿支部大会
• [Presentation] Host responses to probiotic bacteria in C. elegans. (2016)
  • Author(s): Kage-Nakadai, E., Nishikawa, Y.
  • Organizer: 第89回日本細菌学会学術総会
  • Place of Presentation: 大阪国際センター（大阪府、大阪市）
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] A Caenorhabditis elegans homolog of Prox1/Prospero functions in the glia and is required for the development of sensory neurons. (2015)
  • Author(s): Kage-Nakadai, E., Sun, S., Nishikawa, Y., Mitani,
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県、神戸市）
  • Year and Date: 2015-07-30
• [Presentation] A conditional knockout system using single/low-copy transgene integration and deletion mutant strains in Caenorhabditis elegans. (2015)
  • Author(s): E. Kage-Nakadai
  • Organizer: 2015 Japan-Korea International Academic Symposium
  • Place of Presentation: 大阪市立大学（大阪府、大阪市）
  • Year and Date: 2015-03-18 – 2015-03-19
• [Presentation] Approaches to sensory processing mechanisms using Caenorhabditis elegans models. (2015)
  • Author(s): E. Kage-Nakadai
  • Organizer: The 2014 OCARINA Annual International Meeting
  • Place of Presentation: 大阪市立大学（大阪府、大阪市）
  • Year and Date: 2015-03-04 – 2015-03-05
  • Invited
• [Presentation] A conditional knockout system based on the combination of UV/TMP single-copy integration methods and deletion mutant strains in C. elegans. (2014)
  • Author(s): E. Kage-Nakadai, R. Imae, O. Funatsu, S. Hori, Y. Suehiro, S. Yoshina, and S. Mitani
  • Organizer: 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良新公会堂（奈良県、奈良市）
  • Year and Date: 2014-07-14 – 2014-07-17