Elucidation of tumor suppression mechanism through identification of novel proteins involved in mismatch repair-dependent apoptosis

• Project/Area Number: 26830085
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Fukuoka Dental College
• Principal Investigator: Fujikane Ryosuke 福岡歯科大学, 口腔歯学部, 講師 (20581713)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ミスマッチ修復 / アポトーシス / アルキル化剤

Outline of Final Research Achievements

O6-methylguanine (O6meG) can form O6meG/T mispair during DNA replication. The mismatch repair (MMR) complex recognizes this mispair and activates cell cycle checkpoint followed by apoptosis induction. The precise molecular mechanism of this apoptotic pathway is still unclear. In order to unveil molecular mechanism of the apoptotic pathway, we tried to purify proteins which can function or associate with MMR complex. We successfully identified three MMR-associated proteins as new apoptosis-related factors. Analyses of these factors will shed light on understanding of molecular mechanism of tumor suppression and developing new anti-cancer drugs.

Research Products

• [Journal Article] Function of high-mobility groupA proteins in the DNA damage signaling for the induction of apoptosis (2016)
  • Author(s): Fujikane, R., Komori, K., Sekiguchi, M., Hidaka, M.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 31714-31714
  • DOI: 10.1038/srep31714
  • Peer Reviewed / Open Access
• [Presentation] アルキル化剤によって引き起こされるアポトーシスに関わる新規因子の同定と解析 (2016)
  • Author(s): 藤兼亮輔, 関口睦夫, 日高真純
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-10-21
• [Presentation] ミスマッチ修復タンパク質依存のアポトーシス誘導におけるHMGAファミリータンパク質の機能 (2016)
  • Author(s): 藤兼亮輔、武石幸容、関口睦夫、日髙真純
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] O6メチルグアニンによりひき起こされるミスマッチ修復依存のアポトーシス誘導に関わる新規因子の同定 (2015)
  • Author(s): 藤兼亮輔、関口睦夫、日高真純
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Remarks] 学術情報データベース
  • URL: http://www.fdcnet.ac.jp/col/